Recent advancements in soluble expression of recombinant antibody fragments in microbial host systems

P Saroha, RS Patil, AS Rathore - Preparative Biochemistry & …, 2024 - Taylor & Francis
Recombinant fabs dominate the pharmaceutical pipelines today with microbial host systems
continuing to be a major contributor toward their production. Escherichia coli is a versatile …

A De Novo Optimized Cell-Free System for the Expression of Soluble and Active Human Tumor Necrosis Factor-Alpha

NA El-Baky, EM El-Fakharany, SA Sabry, ER El-Helow… - Biology, 2022 - mdpi.com
Simple Summary As a result of increasing demand for the pleiotropic cytokine TNF-α,
recombinant human TNF-α protein with appropriate bioactivities was produced in several …

Identification of Optimal Expression Parameters and Purification of a Codon-Optimized Human GLIS1 Transcription Factor from Escherichia coli

C Dey, V Venkatesan, RP Thummer - Molecular Biotechnology, 2022 - Springer
GLIS1 has multiple roles in embryonic development and in deriving induced pluripotent
stem cells by aiding signaling pathways and chromatin assembly. An inexpensive and …

Active human and murine tumor necrosis factor α cytokines produced from silkworm baculovirus expression system

T Ebihara, J Xu, Y Tonooka, T Nagasato, K Kakino… - Insects, 2021 - mdpi.com
Simple Summary Baculovirus expression vector system (BEVS) is widely employed to
produce eukaryotic recombinant proteins with desired post-translational modifications. The …

Heterologous expression, characterization and evolution prediction of a diaphorase from Geobacillus sp. Y4.1MC1

J Shang, S Yue, F Zeng, Y Chen, L Jia - Biotechnology Letters, 2022 - Springer
Abstract β-hydroxybutyric acid is the most sensitive indicator in ketoacidosis detection, and
accounts for nearly 78% of the ketone bodies. Diaphorase is commonly used to detect the β …

Elucidating signal transduction in multi-domain BLUF photoreceptors by studying the Photoactivated Adenylate Cyclase OaPAC

A Chretien - 2024 - ediss.sub.uni-hamburg.de
Photosensory receptors, essential molecular entities across all domains of life, enable
organisms to detect and respond to light stimuli, underpinning their critical involvement in …