The function of cells in their native habitat often cannot be reliably predicted from genomic
data or from physiology studies of isolates. Traditional experimental approaches to study the …

Viability and metabolic assays are commonly used as proxies to assess the overall
metabolism of microorganisms. The variety of these assays combined with little information …

Dormancy is a bet-hedging strategy used by a wide range of taxa, including
microorganisms. It refers to an organism's ability to enter a reversible state of low metabolic …

Tetrazolium salts have become some of the most widely used tools in cell biology for
measuring the metabolic activity of cells ranging from mammalian to microbial origin. With …

The field of microbiology has traditionally been concerned with and focused on studies at
the population level. Information on how cells respond to their environment, interact with …

The nucleic acid contents of individual bacterial cells as determined with three different
nucleic acid-specific fluorescent dyes (SYBR I, SYBR II, and SYTO 13) and flow cytometry …

Assessing bacterial contamination in environmental samples is critical in determining threats
to public health. The classical methods are time-consuming and only recognize species that …

The development of methodologies for the analysis of microorganisms and microbial
ecology, at the molecular level (ie, nucleic acids, proteins, lipids, and their genes), has …

An issue of critical concern in microbiology is the ability to detect viable but nonculturable
(VBNC) and viable-culturable (VC) cells by methods other than existing approaches. Culture …

The occurrence of viable but non-culturable (VBNC) bacteria will result in significant
underestimation of viable bacterial counts in drinking water. Whereas, much is unknown in …