Our bodies are colonized by a complex ecosystem of bacteria, unicellular eukaryotes and
their viruses that together play a major role in our health. Over the past few years tools …

The theophylline aptamer was isolated from an oligonucleotide library in 1994. Since that
time, the aptamer has found wide utility, particularly in synthetic biology, cellular …

Motility is one of the most important features of life, but its evolutionary origin remains
unknown. In this study, we focused on Spiroplasma, commensal, or parasitic bacteria. They …

The catalytic null mutant of the Cas9 endonuclease from the bacterial CRISPR immune
system, known as dCas9, can be guided by a small RNA to bind DNA sequences of interest …

Most of the current methods for controlling the formation rate of a key protein or enzyme in
cell factories rely on the manipulation of target genes within the pathway. In this article, we …

CRISPR interference (CRISPRi) and CRISPR activation (CRISPRa) have become
ubiquitous approaches to control gene expression in bacteria due to their simple design and …

The tetracycline repressor (TetR) belongs to the most popular, versatile and efficient
transcriptional regulators used in bacterial genetics. In the tetracycline (Tc) resistance …

Genetic perturbation systems are of great interest to redirect metabolic fluxes for value‐
added production, as well as genetic screening for the development of new drugs, or to …

Mycoplasmas are successful pathogens that cause debilitating diseases in humans and
various animal hosts. Despite the exceptionally streamlined genomes, mycoplasmas have …

Transcriptional regulation is essential for maintaining the natural cell metabolism of
microbes and therefore important for metabolic engineering. The development of non-native …