SARS-CoV-2 diagnostics based on nucleic acids amplification: from fundamental concepts to applications and beyond

JM Vindeirinho, E Pinho, NF Azevedo… - Frontiers in cellular and …, 2022 - frontiersin.org
COVID-19 pandemic ignited the development of countless molecular methods for the
diagnosis of SARS-CoV-2 based either on nucleic acid, or protein analysis, with the first …

Point-of-Care diagnostic tools for surveillance of SARS-CoV-2 infections

D Sakthivel, D Delgado-Diaz, L McArthur… - Frontiers in Public …, 2021 - frontiersin.org
Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) is a recently emerged and
highly contagious virus that causes coronavirus disease 2019 (COVID-19). As of August 24 …

Recent advancements in nanosystem-based molecular beacons for RNA detection and imaging

G Yang, T Song, M Wang, M Li, QQ Su… - ACS Applied Nano …, 2022 - ACS Publications
As one of the two nucleic acids used by extant biochemistry, RNA is of great importance for
cellular function and development, and its expression can affect cell homeostasis. Therefore …

Chitosan functionalized gold nanostars as a theranostic platform for intracellular microRNA detection and photothermal therapy

X Dong, Z Zhu, Q Sun, H Zhang, C Yang - Journal of Materials …, 2023 - pubs.rsc.org
The development of a theranostic platform that integrates both diagnostic and therapeutic
capabilities is in great need for precise and personalized medicine. Here, we present a …

Sample-to-answer centrifugal microfluidic droplet PCR platform for quantitation of viral load

L Malic, L Clime, BU Moon, C Nassif, D Da Fonte… - Lab on a Chip, 2024 - pubs.rsc.org
Droplet digital polymerase chain reaction (ddPCR) stands out as a highly sensitive
diagnostic technique that is gaining traction in infectious disease diagnostics due to its …

Graph data science and machine learning for the detection of COVID-19 infection from symptoms

E Alqaissi, F Alotaibi, MS Ramzan - PeerJ Computer Science, 2023 - peerj.com
Background COVID-19 is an infectious disease caused by SARS-CoV-2. The symptoms of
COVID-19 vary from mild-to-moderate respiratory illnesses, and it sometimes requires …

CRISPR/Cas12a-assisted visible fluorescence for pseudo dual nucleic acid detection based on an integrated chip

Y Zhu, J Liu, S Liu, X Zhu, J Wu, Q Zhou, J He… - Analytica Chimica …, 2023 - Elsevier
Background A false negative result is one of the major problems in nucleic acid detection.
Failure to screen positive samples for pathogens or viruses poses a risk to public health …

Unlocking SARS-CoV-2 detection in low-and middle-income countries

R Alcántara, K Peñaranda, G Mendoza-Rojas… - Cell reports …, 2021 - cell.com
Low-and middle-income countries (LMICs) are significantly affected by SARS-CoV-2,
partially due to their limited capacity for local production and implementation of molecular …

Strategies that facilitate extraction-free SARS-CoV-2 nucleic acid amplification tests

DJ Delgado-Diaz, D Sakthivel, HHT Nguyen… - Viruses, 2022 - mdpi.com
The COVID-19 pandemic has resulted in an unprecedented global demand for in vitro
diagnostic reagents. Supply shortages and hoarding have impacted testing capacity which …

A high-performance polymer composite column for coronavirus nucleic acid purification

A Zarouri, AMT Barnes, H Aboubakr… - Scientific reports, 2024 - nature.com
Here, we report the development of a novel polymer composite (PC) purification column and
kit. The performance of the PC columns was compared to conventional silica gel (SG) …