Proteins are large, complex molecules responsible for various biological processes.
However, protein misfolding may lead to various life-threatening diseases. Therefore, it is …

The Chromosome-centric Human Proteome Project (C-HPP) aims to systematically map the
entire human proteome with the intent to enhance our understanding of human biology at …

The Ca2+-sensing protein calmodulin (CaM) is a popular model of biological ion binding
since it is both experimentally tractable and essential to survival in all eukaryotic cells. CaM …

Chemical denaturant titrations can be used to accurately determine protein stability.
However, data acquisition is typically labour intensive, has low throughput and is difficult to …

Raman spectroscopy is an important tool in the study of vibrational properties and
composition of molecules, peptides, and even proteins. Raman spectra can be simulated …

The integration of atomic-resolution experimental and computational methods offers the
potential for elucidating key aspects of protein folding that are not revealed by either …

Common experimental approaches for characterizing structural conversion processes such
as protein folding and self-assembly do not report on all aspects of the evolution from an …

The ability to quantify the local electrostatic environment of proteins and protein/peptide
assemblies is key to gaining a microscopic understanding of many biological interactions …

Thioamides are sterically almost identical to their oxoamide counterparts, but they are
weaker hydrogen bond acceptors. Therefore, thioamide amino acids are excellent …

Trifluoroethanol (TFE) is commonly used to induce protein secondary structure, especially α-
helix formation. Due to its amphiphilic nature, however, TFE can also self-associate to form …