Developing recombinant protein pharmaceuticals has proved to be very challenging
because of both the complexity of protein production and purification, and the limited …

Drying is a commonly used technique for improving the product stability of biotherapeutics.
Typically, drying is accomplished through freeze-drying, as evidenced by the availability of …

Interest in green chemistry and clean processes has grown so much in recent years that
topics such as fluorous biphasic catalysis, metal organic frameworks, and process …

We report that the introduction of low concentrations of intracellular trehalose can greatly
improve the survival of mammalian cells during cryopreservation. Using a genetically …

Objective—To evaluate a portable real-time reverse transcriptase-polymerase chain
reaction (RT-PCR) assay designed to detect all 7 viral serotypes of footand-mouth disease …

Most low water content or frozen food products are partly or fully amorphous. This review will
discuss the extent to which it is possible to understand and predict their behavior during …

Recent studies have demonstrated that molecular mobility is an important factor affecting the
chemical stability of amorphous pharmaceuticals, including small-molecular-weight drugs …

CONTENTS 26.1 Freezing in Biological Materials.............................................................................
..................... 636 26.2 Mode of Preserving Action …

The stabilizing effect of sugar-glass matrix materials for freeze-drying proteins or nucleic
acids has been variously ascribed to the thermodynamic effect of 'water replacement'by …

Sugar glasses are widely used to stabilize proteins during drying and subsequent storage.
To act successfully as a protectant, the sugars should have a high glass transition …