Resistance to rifampicin (RIF) is a broad subject covering not just the mechanism of clinical
resistance, nearly always due to a genetic change in the β subunit of bacterial RNA …

The specificity of DNA-dependent RNA polymerase for target promotes is largely due to the
replaceable sigma subunit that it carries. Multiple sigma proteins, each conferring a unique …

Rifamycins are a class of antibiotics that were first discovered in 1957 and are known for
their use in treating tuberculosis (TB). Rifamycins exhibit bactericidal activity against many …

A 471-base pair HindIII DNA fragment of Bacillus subtilis contains two overlapping
promoters which are recognized in vitro by sigma 55-and sigma 37-containing RNA …

INTRODUCTION Bacillus subtilis is the most widely studied and thoroughly mapped gram-
positive microorganism. Two of the driving forces behind the study of the genetics ofB …

INTRODUCTION Bacillus subtilis is the most widely studied and thoroughly mapped gram-
positive microorganism. Studies have focused on many different aspects of this species …

Low concentrations of the RNA polymerase inhibitor rifampin added to an exponentially
growing culture of Bacillus subtilis led to an instant inhibition of growth. Survival experiments …

The Bacillus subtilis rpoD gene has been sequenced and the primary structure of its product
deduced. The molecular weight calculated for the sigma 43 is 42,828. The DNA and protein …

The DNA-dependent RNA polymerase core enzyme in Gram-positive bacteria consists of
seven subunits. Whilst four of them (α2ββ′) are essential, three smaller subunits, δ, ɛ and …

We have devised a new procedure for the purification of highly active preparations of
Bacillus subtilis RNA polymerase holoenzyme. A column of heparin-agarose A-15m is used …