Cullin-RING ubiquitin ligases (CRLs) are dynamic modular platforms that regulate myriad
biological processes through target-specific ubiquitylation. Our knowledge of this system …

The selective degradation of many short-lived proteins in eukaryotic cells is carried out by
the ubiquitin system. In this pathway, proteins are targeted for degradation by covalent …

SCF complexes are the largest family of E3 ubiquitin–protein ligases and mediate the
ubiquitination of diverse regulatory and signalling proteins. Here we present the crystal …

Cullin proteins are molecular scaffolds that have crucial roles in the post-translational
modification of cellular proteins involving ubiquitin. The mammalian cullin protein family …

We have reconstituted the ubiquitination pathway for the Cdk inhibitor Sic1 using
recombinant proteins. Skp1, Cdc53, and the F-box protein Cdc4 form a complex, SCF Cdc4 …

The concentrations and functions of many eukaryotic proteins are regulated by the ubiquitin
pathway, which consists of ubiquitin activation (E1), conjugation (E2), and ligation (E3) …

▪ Abstract Protein degradation is deployed to modulate the steady-state abundance of
proteins and to switch cellular regulatory circuits from one state to another by abrupt …

Exit from mitosis requires the inactivation of mitotic cyclin-dependent kinases (CDKs) by an
unknown mechanism. We show that the Cdc14 phosphatase triggers mitotic exit by three …

We show that overexpression of Polo-like kinase 4 (Plk4) in human cells induces
centrosome amplification through the simultaneous generation of multiple procentrioles …

Oscillations in the activity of cyclin-dependent kinases (CDKs) promote progression through
the eukaryotic cell cycle. This review examines how proteolysis regulates CDK activity—by …