The AAA+ superfamily: a review of the structural and mechanistic principles of these molecular machines
ATPases associated with diverse cellular activities (AAA+ proteins) are a superfamily of
proteins found throughout all domains of life. The hallmark of this family is a conserved …
proteins found throughout all domains of life. The hallmark of this family is a conserved …
PCNA loaders and unloaders—One ring that rules them all
During each cell duplication, the entirety of the genomic DNA in every cell must be
accurately and quickly copied. Given the short time available for the chore, the requirement …
accurately and quickly copied. Given the short time available for the chore, the requirement …
Cryo-EM structures reveal high-resolution mechanism of a DNA polymerase sliding clamp loader
Sliding clamps are ring-shaped protein complexes that are integral to the DNA replication
machinery of all life. Sliding clamps are opened and installed onto DNA by clamp loader …
machinery of all life. Sliding clamps are opened and installed onto DNA by clamp loader …
Structural mechanisms for replicating DNA in eukaryotes
I Attali, MR Botchan, JM Berger - Annual review of biochemistry, 2021 - annualreviews.org
The faithful and timely copying of DNA by molecular machines known as replisomes
depends on a disparate suite of enzymes and scaffolding factors working together in a …
depends on a disparate suite of enzymes and scaffolding factors working together in a …
DNA is loaded through the 9-1-1 DNA checkpoint clamp in the opposite direction of the PCNA clamp
F Zheng, RE Georgescu, NY Yao… - Nature structural & …, 2022 - nature.com
Abstract The 9-1-1 DNA checkpoint clamp is loaded onto 5′-recessed DNA to activate the
DNA damage checkpoint that arrests the cell cycle. The 9-1-1 clamp is a heterotrimeric ring …
DNA damage checkpoint that arrests the cell cycle. The 9-1-1 clamp is a heterotrimeric ring …
Mechanisms of loading and release of the 9-1-1 checkpoint clamp
JC Castaneda, M Schrecker, D Remus… - Nature structural & …, 2022 - nature.com
Single-stranded or double-stranded DNA junctions with recessed 5′ ends serve as loading
sites for the checkpoint clamp, 9-1-1, which mediates activation of the apical checkpoint …
sites for the checkpoint clamp, 9-1-1, which mediates activation of the apical checkpoint …
[HTML][HTML] Structures of 9-1-1 DNA checkpoint clamp loading at gaps from start to finish and ramification on biology
Rad24-RFC (replication factor C) loads the 9-1-1 checkpoint clamp onto the recessed 5′
ends by binding a 5′ DNA at an external surface site and threading the 3′ single-stranded …
ends by binding a 5′ DNA at an external surface site and threading the 3′ single-stranded …
Cryo-EM structures reveal that RFC recognizes both the 3′-and 5′-DNA ends to load PCNA onto gaps for DNA repair
RFC uses ATP to assemble PCNA onto primed sites for replicative DNA polymerases δ and
ε. The RFC pentamer forms a central chamber that binds 3′ ss/ds DNA junctions to load …
ε. The RFC pentamer forms a central chamber that binds 3′ ss/ds DNA junctions to load …
Structure of the human RAD17–RFC clamp loader and 9–1–1 checkpoint clamp bound to a dsDNA–ssDNA junction
Abstract The RAD9–RAD1–HUS1 (9–1–1) clamp forms one half of the DNA damage
checkpoint system that signals the presence of substantial regions of single-stranded DNA …
checkpoint system that signals the presence of substantial regions of single-stranded DNA …