3DFlex: determining structure and motion of flexible proteins from cryo-EM
Modeling flexible macromolecules is one of the foremost challenges in single-particle
cryogenic-electron microscopy (cryo-EM), with the potential to illuminate fundamental …
cryogenic-electron microscopy (cryo-EM), with the potential to illuminate fundamental …
Methods for cryo-EM single particle reconstruction of macromolecules having continuous heterogeneity
B Toader, FJ Sigworth, RR Lederman - Journal of molecular biology, 2023 - Elsevier
Macromolecules change their shape (conformation) in the process of carrying out their
functions. The imaging by cryo-electron microscopy of rapidly-frozen, individual copies of …
functions. The imaging by cryo-electron microscopy of rapidly-frozen, individual copies of …
Computational methods for single-particle electron cryomicroscopy
A Singer, FJ Sigworth - Annual review of biomedical data …, 2020 - annualreviews.org
Single-particle electron cryomicroscopy (cryo-EM) is an increasingly popular technique for
elucidating the three-dimensional (3D) structure of proteins and other biologically significant …
elucidating the three-dimensional (3D) structure of proteins and other biologically significant …
Cryodrgn2: Ab initio neural reconstruction of 3d protein structures from real cryo-em images
Protein structure determination from cryo-EM data requires reconstructing a 3D volume (or
distribution of volumes) from many noisy and randomly oriented 2D projection images. While …
distribution of volumes) from many noisy and randomly oriented 2D projection images. While …
Amortized inference for heterogeneous reconstruction in cryo-em
Cryo-electron microscopy (cryo-EM) is an imaging modality that provides unique insights
into the dynamics of proteins and other building blocks of life. The algorithmic challenge of …
into the dynamics of proteins and other building blocks of life. The algorithmic challenge of …
Reconstructing continuous distributions of 3D protein structure from cryo-EM images
Cryo-electron microscopy (cryo-EM) is a powerful technique for determining the structure of
proteins and other macromolecular complexes at near-atomic resolution. In single particle …
proteins and other macromolecular complexes at near-atomic resolution. In single particle …
3D flexible refinement: structure and motion of flexible proteins from Cryo-EM
Single particle cryo-EM excels in determining static structures of biological macromolecules
such as proteins. However, many proteins are dynamic, with their motion inherently linked to …
such as proteins. However, many proteins are dynamic, with their motion inherently linked to …
MDSPACE: Extracting continuous conformational landscapes from Cryo-EM single particle datasets using 3D-to-2D flexible fitting based on molecular dynamics …
This article presents an original approach for extracting atomic-resolution landscapes of
continuous conformational variability of biomolecular complexes from cryo electron …
continuous conformational variability of biomolecular complexes from cryo electron …
DeepHEMNMA: ResNet-based hybrid analysis of continuous conformational heterogeneity in cryo-EM single particle images
I Hamitouche, S Jonic - Frontiers in Molecular Biosciences, 2022 - frontiersin.org
Single-particle cryo-electron microscopy (cryo-EM) is a technique for biomolecular structure
reconstruction from vitrified samples containing many copies of a biomolecular complex …
reconstruction from vitrified samples containing many copies of a biomolecular complex …
A Bayesian approach to extracting free-energy profiles from cryo-electron microscopy experiments
Cryo-electron microscopy (cryo-EM) extracts single-particle density projections of individual
biomolecules. Although cryo-EM is widely used for 3D reconstruction, due to its single …
biomolecules. Although cryo-EM is widely used for 3D reconstruction, due to its single …