Expression and Control of Codon-Optimized Granulocyte Colony-Stimulating Factor in Pichia pastoris

N Maity, A Thawani, A Sharma, A Gautam… - Applied biochemistry …, 2016 - Springer
Granulocyte colony-stimulating factor (GCSF) has therapeutic applications due to its proven
efficacy in different forms of neutropenia and chemotherapy-induced leucopenia. The …

Enhancing granulocyte colony-stimulating factor expression in Pichia pastoris through fusion with human serum albumin

M Sigar, N Maity, S Mishra - Preparative Biochemistry and …, 2017 - Taylor & Francis
Protein fusion technology has emerged as one of the important strategies to increase the
level of expression and half-life of therapeutic proteins in heterologous expression systems …

Over expression and analysis of O-glycosylated recombinant human granulocyte colony stimulating factor in Pichia pastoris using Agilent 2100 Bioanalyzer

A Apte-Deshpande, S Somani, G Mandal… - Journal of …, 2009 - Elsevier
Recombinant human granulocyte colony stimulating factor (rhGCSF) was expressed in
methylotrophic yeast Pichia pastoris under the control of AOX1 promoter after integration of …

Human granulocyte colony stimulating factor (G-CSF) produced in the filamentous fungus Aspergillus niger.

N Kraševec, T Milunović, MA Lasnik… - Acta Chimica …, 2014 - search.ebscohost.com
For the first time, a fungal production system is described for expression and secretion of the
medically important human protein G-CSF, in Aspergillus niger. A reliable strategy was …

Construction of a Pichia pastoris strain efficiently producing recombinant human granulocyte-colony stimulating factor (rhG-CSF) and study of its biological activity on …

MB Pykhtina, VP Romanov, SM Miroshnichenko… - Molecular Biology …, 2020 - Springer
Non-glycosylated, recombinant human granulocyte colony-stimulating factor (rhG-CSF),
produced by Escherichia coli (filgrastim, leukostim) is widely used to treat a number of …

Enhanced and Secretory Expression of Human Granulocyte Colony Stimulating Factor by Bacillus subtilis SCK6

S Bashir, S Sadaf, S Ahmad… - BioMed Research …, 2015 - Wiley Online Library
This study describes a simplified approach for enhanced expression and secretion of a
pharmaceutically important human cytokine, that is, granulocyte colony stimulating factor …

[HTML][HTML] Heterologous Expression of Bovine Prochymosin in Pichia pastoris GS115

F Ramezani, S Sadr Mohammad Beigi… - Iranian Journal of …, 2013 - ijbiotech.com
Objectives: In present research we evaluate the expression of this critical enzyme in a
eukaryotic system for future use in cheese industry. Materials and Methods: We have cloned …

Purification of recombinant human granulocyte colony-stimulating factor from Pichia pastoris using two ninta chromatography methods

AM Fuad - IOP Conference Series: Earth and Environmental …, 2020 - iopscience.iop.org
Human granulocyte colony-stimulating factor (hG-CSF) is a glycoprotein that stimulates the
production of mature neutrophil and enhances its survival, proliferation, differentiation, and …

[PDF][PDF] Recombinant human G-CSF production as a protein based drug candidate for hematology and oncology

Y Bozkurt, S Bilgin, S Erden, İF Turan… - International Journal of …, 2019 - dergipark.org.tr
The human granulocyte colony stimulating factor (hG-CSF) is a member of the CSF family.
The first purpose of this study was production of recombinant human G-CSF (rhG-CSF) …

[PDF][PDF] International Journal of Chemistry and Technology

Y BOZKURT, S BİLGİN, SE TAYHAN, İF TURAN… - academia.edu
The human granulocyte colony stimulating factor (hG-CSF) is a member of the CSF family.
The first purpose of this study was production of recombinant human G-CSF (rhG-CSF) …