Visualizing and discovering cellular structures with super-resolution microscopy

YM Sigal, R Zhou, X Zhuang - Science, 2018 - science.org
Super-resolution microscopy has overcome a long-held resolution barrier—the diffraction
limit—in light microscopy and enabled visualization of previously invisible molecular details …

Switchable fluorophores for single-molecule localization microscopy

H Li, JC Vaughan - Chemical reviews, 2018 - ACS Publications
The past decade has witnessed an explosion in the use of super-resolution fluorescence
microscopy methods in biology and other fields. Single-molecule localization microscopy …

Control of intracellular pH and bicarbonate by CO2 diffusion into human sperm

E Grahn, SV Kaufmann, M Askarova, M Ninov… - Nature …, 2023 - nature.com
The reaction of CO2 with H2O to form bicarbonate (HCO3−) and H+ controls sperm motility
and fertilization via HCO3−-stimulated cAMP synthesis. A complex network of signaling …

Molecular basis of human sperm capacitation

LC Puga Molina, GM Luque, PA Balestrini… - Frontiers in cell and …, 2018 - frontiersin.org
In the early 1950s, Austin and Chang independently described the changes that are
required for the sperm to fertilize oocytes in vivo. These changes were originally grouped …

Sperm ion channels and transporters in male fertility and infertility

H Wang, LL McGoldrick, JJ Chung - Nature Reviews Urology, 2021 - nature.com
Mammalian sperm cells must respond to cues originating from along the female
reproductive tract and from the layers of the egg in order to complete their fertilization …

Molecular changes and signaling events occurring in spermatozoa during epididymal maturation

MG Gervasi, PE Visconti - Andrology, 2017 - Wiley Online Library
After leaving the testis, spermatozoa have not yet acquired the ability to move progressively
and are unable to fertilize oocytes. To become fertilization competent, they must go through …

The role of zinc in male fertility

D Allouche-Fitoussi, H Breitbart - International journal of molecular …, 2020 - mdpi.com
Several studies proposed the importance of zinc ion in male fertility. Here, we describe the
properties, roles and cellular mechanisms of action of Zn2+ in spermatozoa, focusing on its …

Structure of a mammalian sperm cation channel complex

S Lin, M Ke, Y Zhang, Z Yan, J Wu - Nature, 2021 - nature.com
The cation channel of sperm (CatSper) is essential for sperm motility and fertility,. CatSper
comprises the pore-forming proteins CATSPER1–4 and multiple auxiliary subunits …

A white paper on phospholipid hydroperoxide glutathione peroxidase (GPx4) forty years later

F Ursini, VB Travain, G Cozza, G Miotto… - Free Radical Biology …, 2022 - Elsevier
The purification of a protein inhibiting lipid peroxidation led to the discovery of the
selenoperoxidase GPx4 forty years ago. Thus, the evidence of the enzymatic activity was …

Comparative analysis of mammalian sperm ultrastructure reveals relationships between sperm morphology, mitochondrial functions and motility

NH Gu, WL Zhao, GS Wang, F Sun - Reproductive Biology and …, 2019 - Springer
Background Sperm morphology mainly refers to the shape of the head, the length of the
flagellar segments, including the midpiece, principal piece and end piece, and the size of …