[HTML][HTML] Design and biocatalytic applications of genetically fused multifunctional enzymes

DT Monterrey, I Ayuso-Fernández, I Oroz-Guinea… - Biotechnology …, 2022 - Elsevier
Fusion proteins, understood as those created by joining two or more genes that originally
encoded independent proteins, have numerous applications in biotechnology, from …

Combination of enzymes and deep eutectic solvents as powerful toolbox for organic synthesis

D Arnodo, E Maffeis, F Marra, S Nejrotti, C Prandi - Molecules, 2023 - mdpi.com
During the last decade, a wide spectrum of applications and advantages in the use of deep
eutectic solvents for promoting organic reactions has been well established among the …

Baeyer–Villiger monooxygenases: tunable oxidative biocatalysts

MJLJ Fürst, A Gran-Scheuch, FS Aalbers… - ACS …, 2019 - ACS Publications
Pollution, accidents, and misinformation have earned the pharmaceutical and chemical
industry a poor public reputation, despite their undisputable importance to society …

[HTML][HTML] Protein scaffolds: a tool for multi-enzyme assembly

S Gad, S Ayakar - Biotechnology Reports, 2021 - Elsevier
The synthesis of complex molecules using multiple enzymes simultaneously in one reaction
vessel has rapidly emerged as a new frontier in the field of bioprocess technology. However …

Metal Affinity Fusion Enzyme Immobilization: Batch Process Showcase for Cascading Alcohol Oxidation and Bayer–Villiger Oxidation in Microaqueous Media

G Vernet, Y Ma, S Serban, A Basso… - ACS Sustainable …, 2024 - ACS Publications
Baeyer–Villiger monooxygenases (BVMOs) show great potential for the selective oxidation
of a wide scope of ketones with exceptional regio-, chemo-, or stereoselectivity to produce …

Genetic fusion of P450 BM3 and formate dehydrogenase towards self-sufficient biocatalysts with enhanced activity

A Kokorin, PD Parshin, PJ Bakkes, AA Pometun… - Scientific reports, 2021 - nature.com
Fusion of multiple enzymes to multifunctional constructs has been recognized as a viable
strategy to improve enzymatic properties at various levels such as stability, activity and …

Design of artificial enzymes bearing several active centers: New trends, opportunities and problems

D Carballares, R Morellon-Sterling… - International Journal of …, 2022 - mdpi.com
Harnessing enzymes which possess several catalytic activities is a topic where intense
research has been carried out, mainly coupled with the development of cascade reactions …

Co‐immobilization and colocalization of multi‐enzyme systems for the cell‐free biosynthesis of aminoalcohols

S Velasco‐Lozano, J Santiago‐Arcos… - …, 2020 - Wiley Online Library
The manufacturing of aminoalcohols is an appealing transformation for industrial
biocatalysis due to their high value as building blocks in synthetic chemistry. By co …

Stabilization of cyclohexanone monooxygenase by computational and experimental library design

MJLJ Fürst, M Boonstra, S Bandstra… - Biotechnology and …, 2019 - Wiley Online Library
Enzymes often by far exceed the activity, selectivity, and sustainability achieved with
chemical catalysts. One of the main reasons for the lack of biocatalysis in the chemical …

Crosslinked aggregates of fusion enzymes in microaqueous organic media

G Vernet, Y Ma, N Zhang, S Kara - ChemBioChem, 2023 - Wiley Online Library
Abstract Baeyer‐Villiger monooxygenases (BVMOs) are attractive for selectively oxidizing
various ketones using oxygen into valuable esters and lactones. However, the application of …