Amplification-free CRISPR/Cas detection technology: challenges, strategies, and perspectives
Rapid and accurate molecular diagnosis is a prerequisite for precision medicine, food
safety, and environmental monitoring. The clustered regularly interspaced short palindromic …
safety, and environmental monitoring. The clustered regularly interspaced short palindromic …
CRISPR‐Cas biochemistry and CRISPR‐based molecular diagnostics
Polymerase chain reaction (PCR)‐based nucleic acid testing has played a critical role in
disease diagnostics, pathogen surveillance, and many more. However, this method requires …
disease diagnostics, pathogen surveillance, and many more. However, this method requires …
Multiplexed CRISPR-based microfluidic platform for clinical testing of respiratory viruses and identification of SARS-CoV-2 variants
Abstract The coronavirus disease 2019 (COVID-19) pandemic has demonstrated a clear
need for high-throughput, multiplexed and sensitive assays for detecting severe acute …
need for high-throughput, multiplexed and sensitive assays for detecting severe acute …
Simplified Cas13-based assays for the fast identification of SARS-CoV-2 and its variants
J Arizti-Sanz, AD Bradley, YB Zhang… - Nature biomedical …, 2022 - nature.com
The widespread transmission and evolution of severe acute respiratory syndrome
coronavirus 2 (SARS-CoV-2) call for rapid nucleic acid diagnostics that are easy to use …
coronavirus 2 (SARS-CoV-2) call for rapid nucleic acid diagnostics that are easy to use …
Programmable CRISPR-Cas9 microneedle patch for long-term capture and real-time monitoring of universal cell-free DNA
B Yang, J Kong, X Fang - Nature communications, 2022 - nature.com
Recent advances in biointerfaces have led to the development of wearable devices that can
provide insights into personal health. As wearable modules, microneedles can extract …
provide insights into personal health. As wearable modules, microneedles can extract …
Amplification‐free detection of SARS‐CoV‐2 and respiratory syncytial virus using CRISPR Cas13a and graphene field‐effect transistors
The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR‐
associated (Cas) systems have recently received notable attention for their applications in …
associated (Cas) systems have recently received notable attention for their applications in …
Engineered LwaCas13a with enhanced collateral activity for nucleic acid detection
Clustered regularly interspaced short palindromic repeats (CRISPR)–CRISPR-associated
protein 13 (Cas13) has been rapidly developed for nucleic-acid-based diagnostics by using …
protein 13 (Cas13) has been rapidly developed for nucleic-acid-based diagnostics by using …
Enzyme kinetics and detector sensitivity determine limits of detection of amplification-free CRISPR-Cas12 and CRISPR-Cas13 diagnostics
DA Huyke, A Ramachandran, VI Bashkirov… - Analytical …, 2022 - ACS Publications
Interest in CRISPR-Cas12 and CRISPR-Cas13 detection continues to increase as these
detection schemes enable the specific recognition of nucleic acids. The fundamental …
detection schemes enable the specific recognition of nucleic acids. The fundamental …
Precise transcript targeting by CRISPR-Csm complexes
D Colognori, M Trinidad, JA Doudna - Nature biotechnology, 2023 - nature.com
Robust and precise transcript targeting in mammalian cells remains a difficult challenge
using existing approaches due to inefficiency, imprecision and subcellular …
using existing approaches due to inefficiency, imprecision and subcellular …
Characterization of a thermostable Cas13 enzyme for one-pot detection of SARS-CoV-2
Type VI CRISPR-Cas systems have been repurposed for various applications such as gene
knockdown, viral interference, and diagnostics. However, the identification and …
knockdown, viral interference, and diagnostics. However, the identification and …