State of the art in CRISPR/Cas system-based signal conversion and amplification applied in the field of food analysis

B Lu, Z Guo, K Zhong, T Osire, Y Sun, L Jiang - Trends in Food Science & …, 2023 - Elsevier
Background Food safety is a major global public health issue. The majority of traditional
culture-based methods have lengthy detection times, require sophisticated sample …

Toward rapid and accurate molecular diagnostics at home

W Liu, LP Lee - Advanced Materials, 2023 - Wiley Online Library
The global outbreaks of infectious diseases have significantly driven an imperative demand
for rapid and accurate molecular diagnostics. Nucleic acid amplification tests (NAATs) …

A SERS-signalled, CRISPR/Cas-powered bioassay for amplification-free and anti-interference detection of SARS-CoV-2 in foods and environmental samples using a …

L Ma, W Zhang, L Yin, Y Li, J Zhuang, L Shen… - Journal of Hazardous …, 2023 - Elsevier
The pandemic of COVID-19 creates an imperative need for sensitive and portable detection
of SARS-CoV-2. We devised a SERS-read, CRISPR/Cas-powered nanobioassay, termed as …

An electrochemical sensing method based on CRISPR/Cas12a system and hairpin DNA probe for rapid and sensitive detection of Salmonella Typhimurium

Y He, F Jia, Y Sun, W Fang, Y Li, J Chen… - Sensors and Actuators B …, 2022 - Elsevier
Abstract Salmonella Typhimurium (S. Typhimurium) is regarded as a major cause of
foodborne diseases, which has been identified as a server threat to public health. Herein, an …

CRISPR-Cas system meets DNA barcoding: Development of a universal nucleic acid test for food authentication

Y Wu, J Liu, H Li, T Zhang, Y Dong, S Deng, Y Lv… - Sensors and Actuators B …, 2022 - Elsevier
Food authenticity is a critical issue owing economic or ethnic considerations. Herein, we
introduce CRISPR-Cas system to food authentication via detecting DNA barcodes of …

Microfluidics: the propellant of CRISPR-based nucleic acid detection

Y Chen, S Qian, X Yu, J Wu, J Xu - Trends in Biotechnology, 2023 - cell.com
Since the discovery of collateral cleavage activity, clustered regularly interspaced short
palindromic repeats (CRISPR)/Cas systems have become the new generation of nucleic …

Applying CRISPR/Cas system as a signal enhancer for DNAzyme-based lead ion detection

Y Chen, H Wu, S Qian, X Yu, H Chen, J Wu - Analytica Chimica Acta, 2022 - Elsevier
Heavy metal lead accumulation in the environment pollutes the ecology systems and further
threatens the human health. It is necessary to develop a sensitive method to detect it. Here …

Multiple crRNAs-assisted CRISPR/Cas12a assay targeting cytochrome b gene for amplification-free detection of meat adulteration

Y Chen, T Yang, S Qian, C Peng, X Wang, T Wang… - Analytica Chimica …, 2022 - Elsevier
Clustered regularly interspaced short palindromic repeats (CRISPR)/Cas systems have
been widely applied in nucleic acid analysis for the high specificity. Coupled with pre …

Unamplified system for sensitive and typing detection of ASFV by the cascade platform that CRISPR-Cas12a combined with graphene field-effect transistor

H Wang, Y Sun, Y Zhou, Y Liu, S Chen, W Sun… - Biosensors and …, 2023 - Elsevier
At present, the 100% case fatality and the cross-infection of virus strains make the ASFV's
harm to society continue to expand. The absence of an effective commercial vaccine poses …

Dipstick-based rapid nucleic acids purification and CRISPR/Cas12a-mediated isothermal amplification for visual detection of African swine fever virus

S Qian, Y Chen, C Peng, X Wang, H Wu, Y Che… - Talanta, 2022 - Elsevier
African swine fever virus (ASFV) can cause highly contagious and fatal disease among
domestic pigs, resulting in considerable economic losses for swine breeders. There is a …