Evolutionary classification of CRISPR–Cas systems: a burst of class 2 and derived variants
The number and diversity of known CRISPR–Cas systems have substantially increased in
recent years. Here, we provide an updated evolutionary classification of CRISPR–Cas …
recent years. Here, we provide an updated evolutionary classification of CRISPR–Cas …
Exploring the trans‐cleavage activity of CRISPR‐Cas12a (cpf1) for the development of a universal electrochemical biosensor
An accurate, rapid, and cost‐effective biosensor for the quantification of disease biomarkers
is vital for the development of early‐diagnostic point‐of‐care systems. The recent discovery …
is vital for the development of early‐diagnostic point‐of‐care systems. The recent discovery …
CRISPR-Cas guides the future of genetic engineering
The diversity, modularity, and efficacy of CRISPR-Cas systems are driving a
biotechnological revolution. RNA-guided Cas enzymes have been adopted as tools to …
biotechnological revolution. RNA-guided Cas enzymes have been adopted as tools to …
[HTML][HTML] Conventional and molecular techniques from simple breeding to speed breeding in crop plants: recent advances and future outlook
In most crop breeding programs, the rate of yield increment is insufficient to cope with the
increased food demand caused by a rapidly expanding global population. In plant breeding …
increased food demand caused by a rapidly expanding global population. In plant breeding …
[HTML][HTML] CRISPR-Cas12a-empowered electrochemical biosensor for rapid and ultrasensitive detection of SARS-CoV-2 delta variant
Abstract Coronavirus disease 2019 (COVID-19) is a highly contagious disease caused by
severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The gold standard method …
severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The gold standard method …
SHERLOCK and DETECTR: CRISPR-Cas systems as potential rapid diagnostic tools for emerging infectious diseases
MI Mustafa, AM Makhawi - Journal of Clinical Microbiology, 2021 - Am Soc Microbiol
Infectious diseases are one of the most intimidating threats to human race, responsible for
an immense burden of disabilities and deaths. Rapid diagnosis and treatment of infectious …
an immense burden of disabilities and deaths. Rapid diagnosis and treatment of infectious …
History of CRISPR-Cas from encounter with a mysterious repeated sequence to genome editing technology
Y Ishino, M Krupovic, P Forterre - Journal of bacteriology, 2018 - Am Soc Microbiol
Clustered regularly interspaced short palindromic repeat (CRISPR)-Cas systems are well-
known acquired immunity systems that are widespread in archaea and bacteria. The RNA …
known acquired immunity systems that are widespread in archaea and bacteria. The RNA …
CRISPR/Cas12a-based technology: A powerful tool for biosensing in food safety
Z Mao, R Chen, X Wang, Z Zhou, Y Peng, S Li… - Trends in Food Science …, 2022 - Elsevier
Background In the context of the current pandemic caused by the novel coronavirus,
molecular detection is not limited to the clinical laboratory, but also faces the challenge of …
molecular detection is not limited to the clinical laboratory, but also faces the challenge of …
[HTML][HTML] Application of different types of CRISPR/Cas-based systems in bacteria
Z Liu, H Dong, Y Cui, L Cong, D Zhang - Microbial cell factories, 2020 - Springer
As important genome editing tools, CRISPR/Cas systems, especially those based on type II
Cas9 and type V Cas12a, are widely used in genetic and metabolic engineering of bacteria …
Cas9 and type V Cas12a, are widely used in genetic and metabolic engineering of bacteria …
Gene editing in plants: progress and challenges
Y Mao, JR Botella, Y Liu, JK Zhu - National Science Review, 2019 - academic.oup.com
The clustered regularly interspaced short palindromic repeat (CRISPR)-associated protein 9
(Cas9) genome editing system is a powerful tool for targeted gene modifications in a wide …
(Cas9) genome editing system is a powerful tool for targeted gene modifications in a wide …