Validation and development of COI metabarcoding primers for freshwater macroinvertebrate bioassessment

V Elbrecht, F Leese - Frontiers in Environmental Science, 2017 - frontiersin.org
A central challenge in the present era of biodiversity loss is to assess and manage human
impacts on freshwater ecosystems. Macroinvertebrates are an important group for such …

Assessing strengths and weaknesses of DNA metabarcoding‐based macroinvertebrate identification for routine stream monitoring

V Elbrecht, EE Vamos, K Meissner… - Methods in Ecology …, 2017 - Wiley Online Library
DNA metabarcoding holds great promise for the assessment of macroinvertebrates in
stream ecosystems. However, few large‐scale studies have compared the performance of …

A new way to contemplate Darwin's tangled bank: how DNA barcodes are reconnecting biodiversity science and biomonitoring

M Hajibabaei, DJ Baird, NA Fahner… - … of the Royal …, 2016 - royalsocietypublishing.org
Encompassing the breadth of biodiversity in biomonitoring programmes has been frustrated
by an inability to simultaneously identify large numbers of species accurately and in a timely …

Macroinvertebrate biomonitoring in Latin America: Progress and challenges

A Cortelezzi, LE Paz - Freshwater Science, 2023 - journals.uchicago.edu
Macroinvertebrates are used as bioindicators worldwide, but the high diversity of
macroinvertebrate species and endemism in Latin America (LA) requires greater knowledge …

Why we need sustainable networks bridging countries, disciplines, cultures and generations for aquatic biomonitoring 2.0: a perspective derived from the DNAqua-Net …

F Leese, A Bouchez, K Abarenkov, F Altermatt… - Advances in ecological …, 2018 - Elsevier
Aquatic biomonitoring has become an essential task in Europe and many other regions as a
consequence of strong anthropogenic pressures affecting the health of lakes, rivers, oceans …

Large-scale biomonitoring of remote and threatened ecosystems via high-throughput sequencing

JF Gibson, S Shokralla, C Curry, DJ Baird, WA Monk… - PloS one, 2015 - journals.plos.org
Biodiversity metrics are critical for assessment and monitoring of ecosystems threatened by
anthropogenic stressors. Existing sorting and identification methods are too expensive and …

Metagenomics versus total RNA sequencing: most accurate data-processing tools, microbial identification accuracy and perspectives for ecological assessments

CA Hempel, N Wright, J Harvie, JS Hleap… - Nucleic Acids …, 2022 - academic.oup.com
Metagenomics and total RNA sequencing (total RNA-Seq) have the potential to improve the
taxonomic identification of diverse microbial communities, which could allow for the …

Freshwater biomonitoring in the Information Age

F Keck, V Vasselon, K Tapolczai… - Frontiers in Ecology …, 2017 - Wiley Online Library
Freshwaters worldwide face serious threats, making their protection increasingly important.
Freshwater monitoring has historically produced valuable data and continues to develop …

Sorting things out: Assessing effects of unequal specimen biomass on DNA metabarcoding

V Elbrecht, B Peinert, F Leese - Ecology and evolution, 2017 - Wiley Online Library
Environmental bulk samples often contain many different taxa that vary several orders of
magnitude in biomass. This can be problematic in DNA metabarcoding and metagenomic …

Is DNA barcoding actually cheaper and faster than traditional morphological methods: results from a survey of freshwater bioassessment efforts in the United States?

ED Stein, MC Martinez, S Stiles, PE Miller… - PloS one, 2014 - journals.plos.org
Taxonomic identification accounts for a substantial portion of cost associated with
bioassessment programs across the United States. New analytical approaches, such as …