In recent years, there has been a dramatic increase in the incidence of hospital-associated
(nosocomial) infections caused by strains of Staphylococcus aureus that are resistant to …

We developed a method for rapidly generating thermostable enzyme variants. Our strategy
is to introduce the gene coding for a given enzyme from a mesophilic organism into a …

Since human immunodeficiency virus (HIV)-specific cell-mediated immune (CMI) responses
are critical in the early control and resolution of HIV infection and correlate with …

MATERIALS AND METHODS Purification and Crystallization Procedures. The KNTase
mutant, D80Y, was expressed in the Escherichia coli overproducing strain BL21 (DE3) …

Chain reaction cloning methods and reagents and kits for performing such methods are
provided. Chain reaction cloning allows ligation of double-stranded DNA molecules by DNA …

The product of a kanamycin resistance gene encoded by plasmid pTB913 isolated from a
thermophilic bacillus was identified as a kanamycin nucleotidyltransferase which is similar to …

The gluconate (gnt) operon of Bacillus subtilis has been cloned and sequenced. Analysis of
the sequence (5482 base pairs) revealed four open reading frames, each of which was …

Revised Manuscript Received August 24, 1993• abstract: Kanamycin nucleotidyltransferase,
as originally isolated from Staphylococcus aureus, inactivates the antibiotic kanamycin by …

The marginal net stability of a folded protein is thought to depend on a small difference
between large, compensating individual forces. Therefore, the net free energy of …

This application is a continuation-in-part of application Ser. No. 08/053,049, filed Apr. 22,
1993, now abandoned, which application is a continuation of application Ser. No …