Neuron tracing from light microscopy images: automation, deep learning and bench testing
Motivation Large-scale neuronal morphologies are essential to neuronal typing, connectivity
characterization and brain modeling. It is widely accepted that automation is critical to the …
characterization and brain modeling. It is widely accepted that automation is critical to the …
Morphological diversity of single neurons in molecularly defined cell types
Dendritic and axonal morphology reflects the input and output of neurons and is a defining
feature of neuronal types,, yet our knowledge of its diversity remains limited. Here, to …
feature of neuronal types,, yet our knowledge of its diversity remains limited. Here, to …
High-throughput imaging: Focusing in on drug discovery in 3D
Abstract 3D organotypic culture models such as organoids and multicellular tumor spheroids
(MCTS) are becoming more widely used for drug discovery and toxicology screening. As a …
(MCTS) are becoming more widely used for drug discovery and toxicology screening. As a …
High-throughput mapping of a whole rhesus monkey brain at micrometer resolution
Whole-brain mesoscale mapping in primates has been hindered by large brain sizes and
the relatively low throughput of available microscopy methods. Here, we present an …
the relatively low throughput of available microscopy methods. Here, we present an …
A platform for brain-wide imaging and reconstruction of individual neurons
The structure of axonal arbors controls how signals from individual neurons are routed
within the mammalian brain. However, the arbors of very few long-range projection neurons …
within the mammalian brain. However, the arbors of very few long-range projection neurons …
Whole-body tissue stabilization and selective extractions via tissue-hydrogel hybrids for high-resolution intact circuit mapping and phenotyping
JB Treweek, KY Chan, NC Flytzanis, B Yang… - Nature protocols, 2015 - nature.com
To facilitate fine-scale phenotyping of whole specimens, we describe here a set of tissue
fixation-embedding, detergent-clearing and staining protocols that can be used to transform …
fixation-embedding, detergent-clearing and staining protocols that can be used to transform …
BigNeuron: large-scale 3D neuron reconstruction from optical microscopy images
Understanding the structure of single neurons is critical for understanding how they function
within neural circuits. BigNeuron is a new community effort that combines modern …
within neural circuits. BigNeuron is a new community effort that combines modern …
[HTML][HTML] NBLAST: rapid, sensitive comparison of neuronal structure and construction of neuron family databases
Neural circuit mapping is generating datasets of tens of thousands of labeled neurons. New
computational tools are needed to search and organize these data. We present NBLAST, a …
computational tools are needed to search and organize these data. We present NBLAST, a …
TeraVR empowers precise reconstruction of complete 3-D neuronal morphology in the whole brain
Neuron morphology is recognized as a key determinant of cell type, yet the quantitative
profiling of a mammalian neuron's complete three-dimensional (3-D) morphology remains …
profiling of a mammalian neuron's complete three-dimensional (3-D) morphology remains …
Live volumetric (4D) visualization and guidance of in vivo human ophthalmic surgery with intraoperative optical coherence tomography
Minimally-invasive microsurgery has resulted in improved outcomes for patients. However,
operating through a microscope limits depth perception and fixes the visual perspective …
operating through a microscope limits depth perception and fixes the visual perspective …