[HTML][HTML] Production of protein-based polymers in Pichia pastoris

MWT Werten, G Eggink, MAC Stuart, FA de Wolf - Biotechnology advances, 2019 - Elsevier
Materials science and genetic engineering have joined forces over the last three decades in
the development of so-called protein-based polymers. These are proteins, typically with …

From small to large-scale: a review of recombinant spider silk and collagen bioproduction

V Gomes, SP Salgueiro - Discover Materials, 2022 - Springer
Advancements in material science have led to the development of new composites and
biomaterials. Spider silk and human collagen are examples of animal-derived polymers for …

Tissue regeneration in vivo within recombinant spidroin 1 scaffolds

MM Moisenovich, O Pustovalova, J Shackelford… - Biomaterials, 2012 - Elsevier
One of the major tasks of tissue engineering is to produce tissue grafts for the replacement
or regeneration of damaged tissue, and natural and recombinant silk-based polymer …

Functionalized silk assembled from a recombinant spider silk fusion protein (Z‐4RepCT) produced in the methylotrophic yeast Pichia pastoris

R Jansson, CH Lau, T Ishida, M Ramström… - Biotechnology …, 2016 - Wiley Online Library
Functional biological materials are a growing research area with potential applicability in
medicine and biotechnology. Using genetic engineering, the possibility to introduce …

3D nanostructural analysis of silk fibroin and recombinant spidroin 1 scaffolds by scanning probe nanotomography

AE Efimov, MM Moisenovich, VG Bogush, II Agapov - RSC Advances, 2014 - pubs.rsc.org
We use serial section scanning probe nanotomography to study the 3D structure,
nanoporosity and pore interconnectivity of biocompatible scaffolds made of fibroin and …

Effect of Recombinant Spidroins Self-Assembly on Rheological Behavior of Their Dispersions and Structure of Electrospun Nanofibrous Materials

TK Tenchurin, RV Sharikov, SI Belousov, DR Streltsov… - Polymers, 2023 - mdpi.com
The effect of primary amino acid sequence in recombinant spidroins on their spatial
organization is crucial for the fabrication of artificial fibers and fibrous materials. This study …

Сравнительный анализ трехмерной наноструктуры пористых биодеградируемых матриксов из рекомбинантного спидроина и фиброина шелка для …

ОИ Агапова, АЕ Ефимов… - Вестник …, 2015 - journal.transpl.ru
Аннотация Цель. Сравнение трехмерной наноструктуры пористых биосовместимых
матриксов из фиброина шелка Bombyx mori и рекомбинантного спидроина rS1/9 …

Биодеградируемые изделия на основе фиброина шелка для тканевой инженерии и регенеративной медицины

ОИ Агапова, ИИ Агапов - 2018 - elibrary.ru
В монографии представлены современные результаты исследования структуры и
биологических свойств изделий на основе фиброина шелка и других …

Comparative analysis of three-dimensional nanostructure of porous biocompatible scaffolds made of recombinant spidroin and silk fibroin for regenerative medicine

OI Agapova, AE Efimov, MM Moisenovich… - Russian Journal of …, 2015 - journal.transpl.ru
Aim. To perform a comparison of three-dimensional nanostructure of porous biocompatible
scaffolds made of fibroin Bombix mori and recombinant spidroin rS1/9. Materials and …

The Development of Bioadhesives Based on Recombinant Analogues of Spider Web Proteins

VG Bogush, LI Davydova, VS Shulyakov… - Applied Biochemistry …, 2022 - Springer
It has been shown that recombinant spidroins rS1/9 and rS2/12 that we previously
developed exhibit adhesive properties with respect to both inorganic and organic substrates …