State-of-the-art tissue-clearing methods provide subcellular-level optical access to intact
tissues from individual organs and even to some entire mammals. When combined with light …

Tissue clearing has become a powerful technique for studying anatomy and morphology at
scales ranging from entire organisms to subcellular features. With the recent proliferation of …

Light-sheet imaging of cleared and expanded samples creates terabyte-sized datasets that
consist of many unaligned three-dimensional image tiles, which must be reconstructed …

Tissue-clearing techniques are powerful tools for biological research and pathological
diagnosis. Here, we describe advanced clear, unobstructed brain imaging cocktails and …

Unbiased quantitative analysis of macroscopic biological samples demands fast imaging
systems capable of maintaining high resolution across large volumes. Here we introduce …

Examination of whole organs with subcellular resolution in health, disease, and during
development is necessary to decipher their biological complexity. However, until recently …

We present a tiling light sheet microscope compatible with all tissue clearing methods for
rapid multicolor 3D imaging of cleared tissues with micron-scale (4× 4× 10 μm 3) to …

Light-sheet fluorescence microscopy (LSFM) is a minimally invasive and high throughput
imaging technique ideal for capturing large volumes of tissue with sub-cellular resolution. A …

Optical recordings of neuronal activity at cellular resolution represent an invaluable tool to
investigate brain mechanisms. Zebrafish larvae is one of the few model organisms where …

Tissue clearing, coupled with immunostaining, enables the transition from two-dimensional
to three-dimensional pathology and has the potential to substantially improve data quality for …