15N‐Labelled proteins by cell‐free protein synthesis: Strategies for high‐throughput NMR studies of proteins and protein–ligand complexes
[15N]‐heteronuclear single quantum coherence (HSQC) spectra provide a readily
accessible fingerprint of [15N]‐labelled proteins, where the backbone amide group of each …
accessible fingerprint of [15N]‐labelled proteins, where the backbone amide group of each …
Advances in nuclear magnetic resonance for drug discovery
R Powers - Expert opinion on drug discovery, 2009 - Taylor & Francis
Background: Drug discovery is a complex and unpredictable endeavor with a high failure
rate. Current trends in the pharmaceutical industry have exacerbated these challenges and …
rate. Current trends in the pharmaceutical industry have exacerbated these challenges and …
Cell-free protein synthesis for analysis by NMR spectroscopy
Cell-free protein synthesis offers fast and inexpensive access to selectively isotope labeled
proteins thath can be measured by NMR spectroscopy in the presence of all the unlabeled …
proteins thath can be measured by NMR spectroscopy in the presence of all the unlabeled …
Quantitative two-dimensional HSQC experiment for high magnetic field NMR spectrometers
H Koskela, O Heikkilä, I Kilpeläinen… - Journal of Magnetic …, 2010 - Elsevier
The finite RF power available on carbon channel in proton–carbon correlation experiments
leads to non-uniform cross peak intensity response across carbon chemical shift range …
leads to non-uniform cross peak intensity response across carbon chemical shift range …
Characterization of de novo synthesized GPCRs supported in nanolipoprotein discs
The protein family known as G-protein coupled receptors (GPCRs) comprises an important
class of membrane-associated proteins, which remains a difficult family of proteins to …
class of membrane-associated proteins, which remains a difficult family of proteins to …
Doubly compensated multiplicity‐edited HSQC experiments utilizing broadband inversion pulses
H Hu, K Krishnamurthy - Magnetic Resonance in Chemistry, 2008 - Wiley Online Library
We propose a family of doubly compensated multiplicity‐edited heteronuclear single
quantum coherence (HSQC) pulse sequences. The key difference between our proposed …
quantum coherence (HSQC) pulse sequences. The key difference between our proposed …
Controlling the diameter, monodispersity, and solubility of ApoA1 nanolipoprotein particles using telodendrimer chemistry
Nanolipoprotein particles (NLPs) are nanometer‐scale discoidal particles that feature a
phospholipid bilayer confined within an apolipoprotein “scaffold,” which are useful for …
phospholipid bilayer confined within an apolipoprotein “scaffold,” which are useful for …
From DNA to protein: No living cells required
M He, Y He, Q Luo, M Wang - Process Biochemistry, 2011 - Elsevier
Proteomics and biotechnology studies require simple and rapid methods to convert the
genetic information into proteins. Whereas heterologous protein expression in living cells is …
genetic information into proteins. Whereas heterologous protein expression in living cells is …
Simultaneous Characterization of a Mixture of Fluorochemicals Using Three-Dimensional 19F−1H Heteronuclear TOCSY Filtered/Edited NMR Experiments
H Hu, P Kulanthaivel… - The Journal of Organic …, 2007 - ACS Publications
Heteronuclear 19F− 1H cross-polarization can be used effectively as a tool for both spectral
filtering and editing in the NMR analysis of the increasing number of fluorine-containing …
filtering and editing in the NMR analysis of the increasing number of fluorine-containing …
Methods and systems for producing nanolipoprotein particles
MA Coleman, PD Hoeprich, BW Segelke - US Patent 11,300,572, 2022 - Google Patents
2018-12-17 Assigned to LAWRENCE LIVERMORE NATIONAL SECURITY, LLC
reassignment LAWRENCE LIVERMORE NATIONAL SECURITY, LLC ASSIGNMENT OF …
reassignment LAWRENCE LIVERMORE NATIONAL SECURITY, LLC ASSIGNMENT OF …