[HTML][HTML] High-content CRISPR screening
CRISPR screens are a powerful source of biological discovery, enabling the unbiased
interrogation of gene function in a wide range of applications and species. In pooled …
interrogation of gene function in a wide range of applications and species. In pooled …
Aptamer-based detection of circulating targets for precision medicine
L Wu, Y Wang, X Xu, Y Liu, B Lin, M Zhang… - Chemical …, 2021 - ACS Publications
The past decade has witnessed ongoing progress in precision medicine to improve human
health. As an emerging diagnostic technique, liquid biopsy can provide real-time …
health. As an emerging diagnostic technique, liquid biopsy can provide real-time …
Challenges in unsupervised clustering of single-cell RNA-seq data
Single-cell RNA sequencing (scRNA-seq) allows researchers to collect large catalogues
detailing the transcriptomes of individual cells. Unsupervised clustering is of central …
detailing the transcriptomes of individual cells. Unsupervised clustering is of central …
MOFA+: a statistical framework for comprehensive integration of multi-modal single-cell data
Technological advances have enabled the profiling of multiple molecular layers at single-
cell resolution, assaying cells from multiple samples or conditions. Consequently, there is a …
cell resolution, assaying cells from multiple samples or conditions. Consequently, there is a …
Ultra-high-throughput single-cell RNA sequencing and perturbation screening with combinatorial fluidic indexing
P Datlinger, AF Rendeiro, T Boenke, M Senekowitsch… - Nature …, 2021 - nature.com
Cell atlas projects and high-throughput perturbation screens require single-cell sequencing
at a scale that is challenging with current technology. To enable cost-effective single-cell …
at a scale that is challenging with current technology. To enable cost-effective single-cell …
Machine learning for integrating data in biology and medicine: Principles, practice, and opportunities
New technologies have enabled the investigation of biology and human health at an
unprecedented scale and in multiple dimensions. These dimensions include a myriad of …
unprecedented scale and in multiple dimensions. These dimensions include a myriad of …
Pooled CRISPR screening with single-cell transcriptome readout
CRISPR-based genetic screens are accelerating biological discovery, but current methods
have inherent limitations. Widely used pooled screens are restricted to simple readouts …
have inherent limitations. Widely used pooled screens are restricted to simple readouts …
Single-cell RNA sequencing: technical advancements and biological applications
Cells are the basic building blocks of organisms and each cell is unique. Single-cell RNA
sequencing has emerged as an indispensable tool to dissect the cellular heterogeneity and …
sequencing has emerged as an indispensable tool to dissect the cellular heterogeneity and …
Uncovering an organ's molecular architecture at single-cell resolution by spatially resolved transcriptomics
Revealing fine-scale cellular heterogeneity among spatial context and the functional and
structural foundations of tissue architecture is fundamental within biological research and …
structural foundations of tissue architecture is fundamental within biological research and …
Challenges in measuring and understanding biological noise
Biochemical reactions are intrinsically stochastic, leading to variation in the production of
mRNAs and proteins within cells. In the scientific literature, this source of variation is typically …
mRNAs and proteins within cells. In the scientific literature, this source of variation is typically …