Nanopore-based technologies beyond DNA sequencing
Inspired by the biological processes of molecular recognition and transportation across
membranes, nanopore techniques have evolved in recent decades as ultrasensitive …
membranes, nanopore techniques have evolved in recent decades as ultrasensitive …
Engineering biological nanopore approaches toward protein sequencing
Biotechnological innovations have vastly improved the capacity to perform large-scale
protein studies, while the methods we have for identifying and quantifying individual proteins …
protein studies, while the methods we have for identifying and quantifying individual proteins …
Unambiguous discrimination of all 20 proteinogenic amino acids and their modifications by nanopore
K Wang, S Zhang, X Zhou, X Yang, X Li, Y Wang… - Nature …, 2024 - nature.com
Natural proteins are composed of 20 proteinogenic amino acids and their post-translational
modifications (PTMs). However, due to the lack of a suitable nanopore sensor that can …
modifications (PTMs). However, due to the lack of a suitable nanopore sensor that can …
Peptide sequencing based on host–guest interaction-assisted nanopore sensing
Y Zhang, Y Yi, Z Li, K Zhou, L Liu, HC Wu - Nature methods, 2024 - nature.com
Direct protein sequencing technologies with improved sensitivity and throughput are still
needed. Here, we propose an alternative method for peptide sequencing based on …
needed. Here, we propose an alternative method for peptide sequencing based on …
Multi-pass, single-molecule nanopore reading of long protein strands
The ability to sequence single protein molecules in their native, full-length form would
enable a more comprehensive understanding of proteomic diversity. Current technologies …
enable a more comprehensive understanding of proteomic diversity. Current technologies …
Translocation of linearized full-length proteins through an engineered nanopore under opposing electrophoretic force
A Sauciuc, B Morozzo della Rocca, MJ Tadema… - Nature …, 2024 - nature.com
Nanopores have recently been used to identify and fingerprint proteins. However, because
proteins, unlike DNA, do not have a uniform charge, the electrophoretic force cannot in …
proteins, unlike DNA, do not have a uniform charge, the electrophoretic force cannot in …
Detection of phosphorylation post-translational modifications along single peptides with nanopores
Current methods to detect post-translational modifications of proteins, such as phosphate
groups, cannot measure single molecules or differentiate between closely spaced …
groups, cannot measure single molecules or differentiate between closely spaced …
Unidirectional single-file transport of full-length proteins through a nanopore
The electrical current blockade of a peptide or protein threading through a nanopore can be
used as a fingerprint of the molecule in biosensor applications. However, threading of full …
used as a fingerprint of the molecule in biosensor applications. However, threading of full …
Resolving isomeric posttranslational modifications using a biological nanopore as a sensor of molecular shape
The chemical nature and precise position of posttranslational modifications (PTMs) in
proteins or peptides are crucial for various severe diseases, such as cancer. State-of-the-art …
proteins or peptides are crucial for various severe diseases, such as cancer. State-of-the-art …
Identification of single amino acid chiral and positional isomers using an electrostatically asymmetric nanopore
Chirality is essential in nearly all biological organizations and chemical reactions but is
rarely considered due to technical limitations in identifying L/D isomerization. Using OmpF, a …
rarely considered due to technical limitations in identifying L/D isomerization. Using OmpF, a …