Single cell photoacoustic microscopy: a review

EM Strohm, MJ Moore, MC Kolios - IEEE Journal of Selected …, 2015 - ieeexplore.ieee.org
Photoacoustic imaging has experienced exponential growth over the past decade, with
many applications in biomedicine. One application ideally suited to the analysis of single …

A tutorial in photoacoustic microscopy and tomography signal processing methods

E Hysi, MJ Moore, EM Strohm, MC Kolios - Journal of Applied Physics, 2021 - pubs.aip.org
Over the last two and a half decades, photoacoustic (PA) imaging has become an important
area of research in biomedical optics. Combining the high contrast of optical imaging with …

Simultaneous acoustic and photoacoustic microfluidic flow cytometry for label-free analysis

V Gnyawali, EM Strohm, JZ Wang, SSH Tsai… - Scientific reports, 2019 - nature.com
We developed a label-free microfluidic acoustic flow cytometer (AFC) based on interleaved
detection of ultrasound backscatter and photoacoustic waves from individual cells and …

Classification of blood cells and tumor cells using label‐free ultrasound and photoacoustics

EM Strohm, MC Kolios - Cytometry Part A, 2015 - Wiley Online Library
A label‐free method that can identify cells in a blood sample using high frequency
photoacoustic and ultrasound signals is demonstrated. When the wavelength of the …

Sizing biological cells using a microfluidic acoustic flow cytometer

EM Strohm, V Gnyawali, JA Sebastian, R Ngunjiri… - Scientific reports, 2019 - nature.com
We describe a new technique that combines ultrasound and microfluidics to rapidly size and
count cells in a high-throughput and label-free fashion. Using 3D hydrodynamic flow …

Properties of cells through life and death–an acoustic microscopy investigation

MM Pasternak, EM Strohm, ESL Berndl, MC Kolios - Cell Cycle, 2015 - Taylor & Francis
Current methods to evaluate the status of a cell are largely focused on fluorescent
identification of molecular biomarkers. The invasive nature of these methods–requiring …

Review of techniques for particle spectroscopy from DC to terahertz frequency

U Dey - IEEE Transactions on Instrumentation and …, 2022 - ieeexplore.ieee.org
Nondestructive, contactless, and label-free characterization of small particles plays an
important role in laboratory research and industrial manufacturing. Also, the analysis of …

Classification of biological cells using a sound wave based flow cytometer

EM Strohm, V Gnyawali… - … and Sensing 2016, 2016 - spiedigitallibrary.org
A flow cytometer that uses sound waves to determine the size of biological cells is
presented. In this system, a microfluidic device made of polydimethylsiloxane (PDMS) was …

Simultaneous ultrasound and photoacoustics based flow cytometry

V Gnyawali, EM Strohm, SSH Tsai… - … : Imaging and Sensing …, 2018 - spiedigitallibrary.org
We have developed a flow cytometer based on simultaneous detection of ultrasound and
photoacoustic waves from individual particles/cells flowing in a microfluidic channel. Our …

Measuring the nucleus-to-cytoplasmic ratio in PC-3 cells using photoacoustic flow cytometry and imaging flow cytometry

JA Sebastian, V Gnyawali, LHY So… - … and Sensing 2019, 2019 - spiedigitallibrary.org
A cell's nucleus-to-cytoplasm (N: C) ratio is a histological metric used to stage malignant
disease. Current N: C assessment methods, such as optical microscopy, are time …