Chemical modifications of CRISPR RNAs to improve gene-editing activity and specificity
E Rozners - Journal of the American Chemical Society, 2022 - ACS Publications
CRISPR (clustered, regularly interspaced, short palindromic repeats) has become a cutting-
edge research method and holds great potential to revolutionize biotechnology and …
edge research method and holds great potential to revolutionize biotechnology and …
Functional DNA regulated CRISPR-Cas12a sensors for point-of-care diagnostics of non-nucleic-acid targets
Beyond its extraordinary genome editing ability, the CRISPR-Cas systems have opened a
new era of biosensing applications due to its high base resolution and isothermal signal …
new era of biosensing applications due to its high base resolution and isothermal signal …
A unified resource for tracking anti-CRISPR names
Dear editors In the battle between CRISPR-Cas* prokaryotic immune systems and the
elements that they target, a diverse array of ''anti-CRISPR''proteins have evolved. These …
elements that they target, a diverse array of ''anti-CRISPR''proteins have evolved. These …
Deciphering off-target effects in CRISPR-Cas9 through accelerated molecular dynamics
CRISPR-Cas9 is the state-of-the-art technology for editing and manipulating nucleic acids.
However, the occurrence of off-target mutations can limit its applicability. Here, all-atom …
However, the occurrence of off-target mutations can limit its applicability. Here, all-atom …
Genetically modified organoids for tissue engineering and regenerative medicine
Q Zhang, J He, D Zhu, Y Chen, M Fu, S Lu, Y Qiu… - Advances in Colloid and …, 2024 - Elsevier
To date, genetically modified organoids are emerging as a promising 3D modeling tool
aimed at solving genetically relevant clinical and biomedical problems for regenerative …
aimed at solving genetically relevant clinical and biomedical problems for regenerative …
Label-free CRISPR/Cas9 assay for site-specific nucleic acid detection
J Hu, M Jiang, R Liu, Y Lv - Analytical chemistry, 2019 - ACS Publications
The development of the clustered regularly interspaced short palindromic repeat
(CRISPR)/Cas9 system has become a revolutionary step for genome engineering because it …
(CRISPR)/Cas9 system has become a revolutionary step for genome engineering because it …
Exploring the catalytic mechanism of Cas9 using information inferred from endonuclease VII
Elucidating the nature of the gene editing mechanism of CRISPR (Clustered Regularly
Interspaced Short Palindromic Repeats) is an important task in view of the role of this …
Interspaced Short Palindromic Repeats) is an important task in view of the role of this …
CRISPR/Cas12a System coupling polyfluorene nanoreporter enriched by magnetic bead-based high-efficiency DNA nanocarrier for fluorescence analysis
J Wei, Y Zhang, S Liu, J Zhao, R Yuan… - Sensors and Actuators B …, 2023 - Elsevier
Currently reported CRISPR/Cas12a-based fluorescence systems are usually constructed
with fluorescent dyes as reporter probes, which are performed the detection in …
with fluorescent dyes as reporter probes, which are performed the detection in …
Programming PAM antennae for efficient CRISPR-Cas9 DNA editing
Bacterial CRISPR-Cas9 nucleases have been repurposed as powerful genome editing
tools. Whereas engineering guide RNAs or Cas nucleases have proven to improve the …
tools. Whereas engineering guide RNAs or Cas nucleases have proven to improve the …
Terminal uridylyl transferase mediated site-directed access to clickable chromatin employing CRISPR-dCas9
Locus-specific interrogation of target genes employing functional probes such as proteins
and small molecules is paramount in decoding the molecular basis of gene function and …
and small molecules is paramount in decoding the molecular basis of gene function and …