Better Cryo-EM specimen preparation: how to deal with the air–water Interface?
N Liu, HW Wang - Journal of Molecular Biology, 2023 - Elsevier
Cryogenic electron microscopy (cryo-EM) is now one of the most powerful and widely used
methods to determine high-resolution structures of macromolecules. A major bottleneck of …
methods to determine high-resolution structures of macromolecules. A major bottleneck of …
Structural basis of inhibition of the human SGLT2–MAP17 glucose transporter
Human sodium–glucose cotransporter 2 (hSGLT2) mediates the reabsorption of the majority
of filtrated glucose in the kidney. Pharmacological inhibition of hSGLT2 by oral small …
of filtrated glucose in the kidney. Pharmacological inhibition of hSGLT2 by oral small …
Structure of the human Mediator-bound transcription preinitiation complex
Eukaryotic transcription requires the assembly of a multisubunit preinitiation complex (PIC)
composed of RNA polymerase II (Pol II) and the general transcription factors. The coactivator …
composed of RNA polymerase II (Pol II) and the general transcription factors. The coactivator …
Structural basis of long-range to short-range synaptic transition in NHEJ
DNA double-strand breaks (DSBs) are a highly cytotoxic form of DNA damage and the
incorrect repair of DSBs is linked to carcinogenesis,. The conserved error-prone non …
incorrect repair of DSBs is linked to carcinogenesis,. The conserved error-prone non …
Structures of the holo CRISPR RNA-guided transposon integration complex
CRISPR-associated transposons (CAST) are programmable mobile genetic elements that
insert large DNA cargos using an RNA-guided mechanism,–. CAST elements contain …
insert large DNA cargos using an RNA-guided mechanism,–. CAST elements contain …
Template and target-site recognition by human LINE-1 in retrotransposition
The long interspersed element-1 (LINE-1, hereafter L1) retrotransposon has generated
nearly one-third of the human genome and serves as an active source of genetic diversity …
nearly one-third of the human genome and serves as an active source of genetic diversity …
Structure of human phagocyte NADPH oxidase in the resting state
Phagocyte oxidase plays an essential role in the first line of host defense against pathogens.
It oxidizes intracellular NADPH to reduce extracellular oxygen to produce superoxide anions …
It oxidizes intracellular NADPH to reduce extracellular oxygen to produce superoxide anions …
Structural mechanism of SGLT1 inhibitors
Y Niu, W Cui, R Liu, S Wang, H Ke, X Lei… - Nature …, 2022 - nature.com
Sodium glucose co-transporters (SGLT) harness the electrochemical gradient of sodium to
drive the uphill transport of glucose across the plasma membrane. Human SGLT1 (hSGLT1) …
drive the uphill transport of glucose across the plasma membrane. Human SGLT1 (hSGLT1) …
Cryo-EM visualization of DNA-PKcs structural intermediates in NHEJ
DNA double-strand breaks (DSBs), one of the most cytotoxic forms of DNA damage, can be
repaired by the tightly regulated nonhomologous end joining (NHEJ) machinery (Stinson …
repaired by the tightly regulated nonhomologous end joining (NHEJ) machinery (Stinson …
Structure of the human SAGA coactivator complex
The SAGA complex is a regulatory hub involved in gene regulation, chromatin modification,
DNA damage repair and signaling. While structures of yeast SAGA (ySAGA) have been …
DNA damage repair and signaling. While structures of yeast SAGA (ySAGA) have been …