Highlights•Cell-free expression is a highly multidisciplinary bioengineering technology.•Cell-
free expression is fast, quantitative and a safe synthetic biology environment.•TXTL …

Cell-free protein synthesis (CFPS) is a technology for the production of proteins in vitro,
which has emerged as a robust platform for biotechnology and synthetic biology …

Bottom-up synthetic biology aims to engineer artificial cells capable of responsive behaviors
by using a minimal set of molecular components. An important challenge toward this goal is …

Living cells have the ability to control the dynamics of responsive assemblies such as the
cytoskeleton by temporally activating and deactivating inert precursors. While DNA …

The pace of change in chemical and biological research enabled by improved detection
systems demands fundamental liquid handling and sample preparation changes. The …

The cell-free system is a tool that harnesses the complex biological processes outside of
living cells. Owing to the system's superior flexibility and manipulability, it has matured its …

Following infection of bacterial cells, bacteriophage modulate double-stranded DNA break
repair pathways to protect themselves from host immunity systems and prioritise their own …

Synthetic biology integrates diverse engineering disciplines to create novel biological
systems for biomedical and technological applications. The substantial growth of the …

Cell-free transcription–translation (TXTL) has recently emerged as a versatile technology to
engineer biological systems. In this chapter, we show how an all E. coli TXTL system can be …

The analysis and improvement of DNA nanostructure biostability is one of the keys areas of
progress needed in DNA nanotechnology applications. Here, we present a plate-compatible …