Trehalose in cryopreservation. Applications, mechanisms and intracellular delivery opportunities

A Murray, P Kilbride, MI Gibson - RSC Medicinal Chemistry, 2024 - pubs.rsc.org
Cryopreservation is crucial to fields including immune and stem cell therapies, reproductive
technology, blood banking, regenerative medicine and across all biotechnology. During …

Bioinspired cryoprotectants enabled by binary natural deep eutectic solvents for sustainable and green cryopreservation

Y Tian, DW Sun, L Xu, TH Fan… - ACS Sustainable …, 2022 - ACS Publications
Cryopreservation plays an important role in prolonging the cell viability, but non-negligible
cytotoxicity of cryoprotectants restrains its further development in many areas with safety …

Fat body glycogen serves as a metabolic safeguard for the maintenance of sugar levels in Drosophila

T Yamada, O Habara, H Kubo, T Nishimura - Development, 2018 - journals.biologists.com
Adapting to changes in food availability is a central challenge for survival. Glucose is an
important resource for energy production, and therefore many organisms synthesize and …

[HTML][HTML] Green Natural Deep Eutectic Agents (GNDEAs) for Biocryopreservation: Syntheses, Properties and Advanced Applications

R Hu, DW Sun, Y Tian - Trends in Food Science & Technology, 2024 - Elsevier
Background Freezing, as a common method of food preservation, has been widely used in
the food industry. However, with further research, the drawbacks it brings (such as ice crystal …

How do animals survive extreme temperature amplitudes? The role of natural deep eutectic solvents

A Gertrudes, R Craveiro, Z Eltayari… - ACS Sustainable …, 2017 - ACS Publications
Recent findings have reported the reason why some living beings are able to withstand the
huge thermal amplitudes between winter and summer in their natural habitats. They are able …

Red blood cell cryopreservation with minimal post-thaw lysis enabled by a synergistic combination of a cryoprotecting polyampholyte with DMSO/trehalose

A Murray, TR Congdon, RMF Tomás… - …, 2021 - ACS Publications
From trauma wards to chemotherapy, red blood cells are essential in modern medicine.
Current methods to bank red blood cells typically use glycerol (40 wt%) as a cryoprotective …

Thermal tolerance limits of diamondback moth in ramping and plunging assays

C Nguyen, MH Bahar, G Baker, NR Andrew - PLoS One, 2014 - journals.plos.org
Thermal sensitivity is a crucial determinant of insect abundance and distribution. The way it
is measured can have a critical influence on the conclusions made. Diamondback moth …

Hemolymph metabolites and osmolality are tightly linked to cold tolerance of Drosophila species: a comparative study

T Olsson, HA MacMillan, N Nyberg… - Journal of …, 2016 - journals.biologists.com
Drosophila, like most insects, are susceptible to low temperatures, and will succumb to
temperatures above the freezing point of their hemolymph. For these insects, cold exposure …

Profiling of urinary amino-carboxylic metabolites by in-situ heptafluorobutyl chloroformate mediated sample preparation and gas chromatography–mass spectrometry

P Hušek, Z Švagera, D Hanzlíková… - … of Chromatography A, 2016 - Elsevier
Abstract A novel 1, 1, 1, 2, 2, 3, 3-heptafluorobutyl chloroformate reagent (HFBCF) was
examined for in-situ derivatization of amino-carboxylic metabolites in human urine. The …

Non-destructive hyperspectral imaging and machine learning-based predictive models for physicochemical quality attributes of apples during storage as affected by …

AY Khaled, N Ekramirad, KD Donohue, RT Villanueva… - Agriculture, 2023 - mdpi.com
The demand for high-quality apples remains strong throughout the year, as they are one of
the top three most popular fruits globally. However, the apple industry faces challenges in …