PRC2 is high maintenance
As the process that silences gene expression ensues during development, the stage is set
for the activity of Polycomb-repressive complex 2 (PRC2) to maintain these repressed gene …
for the activity of Polycomb-repressive complex 2 (PRC2) to maintain these repressed gene …
Polycomb gene silencing mechanisms: PRC2 chromatin targeting, H3K27me3'readout', and phase separation-based compaction
Modulation of chromatin structure and/or modification by Polycomb repressive complexes
(PRCs) provides an important means to partition the genome into functionally distinct …
(PRCs) provides an important means to partition the genome into functionally distinct …
Polycomb condensates can promote epigenetic marks but are not required for sustained chromatin compaction
JM Eeftens, M Kapoor, D Michieletto… - Nature …, 2021 - nature.com
Organization of the genome into transcriptionally active euchromatin and silenced
heterochromatin is essential for eukaryotic cell function. Phase-separation has been …
heterochromatin is essential for eukaryotic cell function. Phase-separation has been …
Phase-separated transcriptional condensates accelerate target-search process revealed by live-cell single-molecule imaging
Compartmentalization by liquid-liquid phase separation is implicated in transcription. It
remains unclear whether and how transcriptional condensates accelerate the search of …
remains unclear whether and how transcriptional condensates accelerate the search of …
Histone variant and cell context determine H3K27M reprogramming of the enhancer landscape and oncogenic state
S Nagaraja, MA Quezada, SM Gillespie, M Arzt… - Molecular cell, 2019 - cell.com
Development of effective targeted cancer therapies is fundamentally limited by our
molecular understanding of disease pathogenesis. Diffuse intrinsic pontine glioma (DIPG) is …
molecular understanding of disease pathogenesis. Diffuse intrinsic pontine glioma (DIPG) is …
Multiple modes of PRC2 inhibition elicit global chromatin alterations in H3K27M pediatric glioma
A methionine substitution at lysine-27 on histone H3 variants (H3K27M) characterizes~ 80%
of diffuse intrinsic pontine gliomas (DIPG) and inhibits polycomb repressive complex 2 …
of diffuse intrinsic pontine gliomas (DIPG) and inhibits polycomb repressive complex 2 …
Transcription dynamics and genome organization in the mammalian nucleus: Recent advances
K Wagh, DA Stavreva, GL Hager - Molecular Cell, 2024 - cell.com
Single-molecule tracking (SMT) has emerged as the dominant technology to investigate the
dynamics of chromatin-transcription factor (TF) interactions. How long a TF needs to bind to …
dynamics of chromatin-transcription factor (TF) interactions. How long a TF needs to bind to …
Spatiotemporal coordination of transcription preinitiation complex assembly in live cells
Transcription initiation by RNA polymerase II (RNA Pol II) requires preinitiation complex
(PIC) assembly at gene promoters. In the dynamic nucleus, where thousands of promoters …
(PIC) assembly at gene promoters. In the dynamic nucleus, where thousands of promoters …
Histone H3 mutations: an updated view of their role in chromatin deregulation and cancer
BR Lowe, LA Maxham, JJ Hamey, MR Wilkins… - Cancers, 2019 - mdpi.com
In this review, we describe the attributes of histone H3 mutants identified in cancer. H3
mutants were first identified in genes encoding H3. 3, in pediatric high-grade glioma, and …
mutants were first identified in genes encoding H3. 3, in pediatric high-grade glioma, and …
Kinetic principles underlying pioneer function of GAGA transcription factor in live cells
How pioneer factors interface with chromatin to promote accessibility for transcription control
is poorly understood in vivo. Here, we directly visualize chromatin association by the …
is poorly understood in vivo. Here, we directly visualize chromatin association by the …