[HTML][HTML] Recent trends in two-dimensional liquid chromatography

RS van den Hurk, M Pursch, DR Stoll… - TrAC Trends in Analytical …, 2023 - Elsevier
Multi-dimensional liquid chromatography (MD-LC) continues to gain in popularity for
applications where conventional one-dimensional liquid chromatography is insufficient to …

Proteomics of human spermatozoa

J Castillo, A de la Iglesia, M Leiva, M Jodar… - Human …, 2023 - academic.oup.com
Proteomic methodologies offer a robust approach to identify and quantify thousands of
proteins from semen components in both fertile donors and infertile patients. These …

Regulatory T cells play a crucial role in maintaining sperm tolerance and male fertility

F Barrachina, K Ottino, ML Elizagaray… - Proceedings of the …, 2023 - National Acad Sciences
Regulatory T cells (Tregs) modulate tissue homeostatic processes and immune responses.
Understanding tissue-Treg biology will contribute to developing precision-targeting …

Differences in the proteome of stallion spermatozoa explain stallion-to-stallion variability in sperm quality post-thaw

G Gaitskell-Phillips, FE Martín-Cano… - Biology of …, 2021 - academic.oup.com
The identification of stallions and or ejaculates that will provide commercially acceptable
quality post-thaw before cryopreservation is of great interest, avoiding wasting time and …

Mapping the human sperm proteome–novel insights into reproductive research

MA Miyazaki, RL Guilharducci, P Intasqui… - Expert Review of …, 2023 - Taylor & Francis
Introduction Spermatozoa are highly specialized cells with unique morphology. In addition,
spermatozoa lose a considerable amount of cytoplasm during spermiogenesis, when they …

TMT-based proteomic analysis of human spermatozoa from unexplained recurrent miscarriage patients before and after oral antioxidant treatment

A Fernandez-Encinas, J Ribas-Maynou, A García-Peiró… - Biomedicines, 2022 - mdpi.com
Recently, sperm quality and the presence of double-stranded breaks (DSB) has been
pointed out as a possible cause of recurrent miscarriage, and the use of antioxidants has …

What does intracytoplasmic sperm injection change in embryonic development? The spermatozoon contribution

S Chamayou, F Giacone, R Cannarella… - Journal of Clinical …, 2023 - mdpi.com
The intracytoplasmic sperm injection (ICSI) technique was invented to solve severe male
infertility due to altered sperm parameters. Nowadays, it is applied worldwide for the …

Biomarker-based human and animal sperm phenotyping: the good, the bad and the ugly

P Sutovsky, LE Hamilton, M Zigo… - Biology of …, 2024 - academic.oup.com
Conventional, brightfield-microscopic semen analysis provides important baseline
information about sperm quality of an individual; however, it falls short of identifying subtle …

Sperm cryopreservation impacts the early development of equine embryos by downregulating specific transcription factors

JM Ortiz-Rodríguez, FE Martín-Cano FE… - BioRxiv, 2021 - biorxiv.org
Equine embryos were obtained by insemination with either fresh or frozen-thawed
spermatozoa at 8, 10 and 12 h post spontaneous ovulation, maintaining the pairs mare …

Sperm preparedness and adaptation to osmotic and pH stressors relate to functional competence of sperm in Bos taurus

M Lavanya, SS Archana, D Swathi, L Ramya… - Scientific Reports, 2021 - nature.com
The adaptive ability of sperm in the female reproductive tract micromilieu signifies the
successful fertilization process. The study aimed to analyze the preparedness of sperm to …