The physiological importance of cholesterol in the cell plasma membrane has attracted
increased attention in recent years. Consequently, the use of methods of controlled …

We review the main trends in the development of fluorescence probes to obtain information
about the structure, dynamics, and interactions in biomembranes. These probes are efficient …

The success of fluorescence experiments requires attention to experimental details and an
understanding of the instrumentation. There are also many potential artifacts that can distort …

Exosomes are small vesicles secreted from multivesicular bodies, which are able to
stimulate the immune system leading to tumour cell eradication. We have analysed lipids of …

The membrane raft hypothesis postulates the existence of lipid bilayer membrane
heterogeneities, or domains, supposed to be important for cellular function, including lateral …

Cholesterol and sphingomyelin form together a highly ordered membrane phase, which is
believed to play important biological functions in plasma membranes of mammalian cells …

Fluorescence microscopy imaging is an important technique for studying lipid membranes
and is increasingly being used for examining lipid bilayer membranes, especially those …

We demonstrate critical behavior in giant plasma membrane vesicles (GPMVs) that are
isolated directly from living cells. GPMVs contain two liquid phases at low temperatures and …

A biological membrane is conceptualized as a system in which membrane proteins are
naturally matched to the equilibrium thickness of the lipid bilayer. Cholesterol, in addition to …

Abstract We use 2 H-NMR, 1 H-MAS NMR, and fluorescence microscopy to detect
immiscibility in three particular phospholipid ratios mixed with 30% cholesterol: 2: 1 …