Current molecular diagnostics of human pathogens provide limited information that is often
not sufficient for outbreak and transmission investigation. Next generation sequencing …

Abstract Next Generation Sequencing (NGS) combined with powerful bioinformatic
approaches are revolutionising food microbiology. Whole genome sequencing (WGS) of …

Whole genome sequencing (WGS) shows great potential for real-time monitoring and
identification of infectious disease outbreaks. However, rapid and reliable comparison of …

Multilocus sequence typing (MLST) was proposed in 1998 as a portable sequence-based
method for identifying clonal relationships among bacteria. Today, in the whole-genome era …

Genomics and whole genome sequencing (WGS) have the capacity to greatly enhance
knowledge and understanding of infectious diseases and clinical microbiology. The growth …

Characterizing the errors generated by common high-throughput sequencing platforms and
telling true genetic variation from technical artefacts are two interdependent steps, essential …

Whole-genome sequencing (WGS) has emerged today as an ultimate typing tool to
characterize Listeria monocytogenes outbreaks. However, data analysis and interlaboratory …

The exploration of microbial communities by sequencing 16S rRNA genes has expanded
with low-cost, high-throughput sequencing instruments. Illumina-based 16S rRNA gene …

In nature microorganisms live in complex microbial communities. Comprehensive taxonomic
and functional knowledge about microbial communities supports medical and technical …

Multidrug-resistant nosocomial pathogens present a major burden for hospitals. Rapid
cluster identification and pathogen profiling, ie, of antibiotic resistance and virulence genes …