Serial femtosecond crystallography

TRM Barends, B Stauch, V Cherezov… - Nature reviews Methods …, 2022 - nature.com
With the advent of X-ray free-electron lasers (XFELs), new, high-throughput serial
crystallography techniques for macromolecular structure determination have emerged …

Radiation damage to biological macromolecules∗

EF Garman, M Weik - Current Opinion in Structural Biology, 2023 - Elsevier
In this review, we describe recent research developments into radiation damage effects in
macromolecular X-ray crystallography observed at synchrotrons and X-ray free electron …

X-ray-based techniques to study the nano–bio interface

C Sanchez-Cano, RA Alvarez-Puebla, JM Abendroth… - ACS …, 2021 - ACS Publications
X-ray-based analytics are routinely applied in many fields, including physics, chemistry,
materials science, and engineering. The full potential of such techniques in the life sciences …

Macromolecular room temperature crystallography

M Fischer - Quarterly Reviews of Biophysics, 2021 - cambridge.org
X-ray crystallography enables detailed structural studies of proteins to understand and
modulate their function. Conducting crystallographic experiments at cryogenic temperatures …

Serial protein crystallography in an electron microscope

R Bücker, P Hogan-Lamarre, P Mehrabi… - Nature …, 2020 - nature.com
Serial X-ray crystallography at free-electron lasers allows to solve biomolecular structures
from sub-micron-sized crystals. However, beam time at these facilities is scarce, and …

Characterization of dye-decolorizing peroxidase from Bacillus subtilis

P Dhankhar, V Dalal, JK Mahto, BR Gurjar… - Archives of Biochemistry …, 2020 - Elsevier
The dye-decolorizing peroxidases (DyPs) belong to a unique heme peroxidase family for
their biotechnological potential to detoxify synthetic dyes. In this work, we have …

Time-resolved serial crystallography to track the dynamics of carbon monoxide in the active site of cytochrome c oxidase

C Safari, S Ghosh, R Andersson, J Johannesson… - Science …, 2023 - science.org
Cytochrome c oxidase (C c O) is part of the respiratory chain and contributes to the
electrochemical membrane gradient in mitochondria as well as in many bacteria, as it uses …

2′-O methylation of RNA cap in SARS-CoV-2 captured by serial crystallography

M Wilamowski, DA Sherrell… - Proceedings of the …, 2021 - National Acad Sciences
The genome of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)
coronavirus has a capping modification at the 5′-untranslated region (UTR) to prevent its …

Radiation damage and dose limits in serial synchrotron crystallography at cryo-and room temperatures

E de La Mora, N Coquelle, CS Bury… - Proceedings of the …, 2020 - National Acad Sciences
Radiation damage limits the accuracy of macromolecular structures in X-ray crystallography.
Cryogenic (cryo-) cooling reduces the global radiation damage rate and, therefore, became …

[PDF][PDF] Best practices for time-resolved serial synchrotron crystallography

EC Schulz, BA Yorke, AR Pearson… - … Section D: Structural …, 2022 - journals.iucr.org
With recent developments in X-ray sources, instrumentation and data-analysis tools, time-
resolved crystallographic experiments, which were originally the preserve of a few expert …