PCR past, present and future

H Zhu, H Zhang, Y Xu, S Laššáková, M Korabečná… - …, 2020 - Taylor & Francis
PCR has become one of the most valuable techniques currently used in bioscience,
diagnostics and forensic science. Here we review the history of PCR development and the …

Loop-mediated isothermal amplification (LAMP)–review and classification of methods for sequence-specific detection

L Becherer, N Borst, M Bakheit, S Frischmann… - Analytical …, 2020 - pubs.rsc.org
In the course of the last 20 years, isothermal nucleic acid amplification tests have emerged
as an important diagnostic tool, not only for clinical applications, but also for food quality …

Molecular diagnosis of COVID-19: challenges and research needs

W Feng, AM Newbigging, C Le, B Pang… - Analytical …, 2020 - ACS Publications
Molecular diagnosis of COVID-19 primarily relies on the detection of RNA of the SARS-CoV-
2 virus, the causative infectious agent of the pandemic. Reverse transcription polymerase …

Pediatric low-grade glioma in the era of molecular diagnostics

S Ryall, U Tabori, C Hawkins - Acta neuropathologica communications, 2020 - Springer
Low grade gliomas are the most frequent brain tumors in children and encompass a
spectrum of histologic entities which are currently assigned World Health Organisation …

Droplet digital PCR of viral‎ DNA/RNA, current progress, challenges, and future perspectives

AA Kojabad, M Farzanehpour… - Journal of Medical …, 2021 - Wiley Online Library
High‐throughput droplet‐based digital PCR (ddPCR) is a refinement of the conventional
polymerase chain reaction (PCR)‎ methods. In ddPCR, DNA/RNA is encapsulated …

Digital CRISPR/Cas‐Assisted assay for rapid and sensitive detection of SARS‐CoV‐2

JS Park, K Hsieh, L Chen, A Kaushik… - Advanced …, 2021 - Wiley Online Library
The unprecedented demand for rapid diagnostics in response to the COVID‐19 pandemic
has brought the spotlight onto clustered regularly interspaced short palindromic repeats …

The clinical potential of circulating microRNAs in obesity

C Ji, X Guo - Nature Reviews Endocrinology, 2019 - nature.com
Obesity is a complex condition that is characterized by excessive fat accumulation, which
can lead to the development of metabolic disorders, such as type 2 diabetes mellitus …

Droplet-based digital PCR (ddPCR) and its applications

Y Hou, S Chen, Y Zheng, X Zheng, JM Lin - TrAC Trends in Analytical …, 2023 - Elsevier
As a new generation absolute quantitive PCR technique, droplet-based digital PCR
(ddPCR) provides the benefits of high sensitivity, precision, and reproducibility. A low-cost …

Helicobacter pylori infection, its laboratory diagnosis, and antimicrobial resistance: a perspective of clinical relevance

S Ansari, Y Yamaoka - Clinical microbiology reviews, 2022 - Am Soc Microbiol
Despite the recent decrease in overall prevalence of Helicobacter pylori infection, morbidity
and mortality rates associated with gastric cancer remain high. The antimicrobial resistance …

Point-of-care PCR assays for COVID-19 detection

N Gupta, S Augustine, T Narayan, A O'Riordan, A Das… - Biosensors, 2021 - mdpi.com
Molecular diagnostics has been the front runner in the world's response to the COVID-19
pandemic. Particularly, reverse transcriptase-polymerase chain reaction (RT-PCR) and the …