Partitioning in aqueous two‐phase systems: Analysis of strengths, weaknesses, opportunities and threats

RRG Soares, AM Azevedo, JM Van Alstine… - Biotechnology …, 2015 - Wiley Online Library
For half a century aqueous two‐phase systems (ATPSs) have been applied for the extraction
and purification of biomolecules. In spite of their simplicity, selectivity, and relatively low cost …

Emerging biotechnology applications of aqueous two‐phase systems

AG Teixeira, R Agarwal, KR Ko… - Advanced …, 2018 - Wiley Online Library
Liquid–liquid phase separation between aqueous solutions containing two incompatible
polymers, a polymer and a salt, or a polymer and a surfactant, has been exploited for a wide …

Multimodal chromatography: an efficient tool in downstream processing of proteins

K Kallberg, HO Johansson, L Bulow - Biotechnology journal, 2012 - Wiley Online Library
Chromatography has become an indispensable tool for the purification of proteins. Since the
regulatory demands on protein purity are expected to become stricter, the need for …

Aqueous biphasic extraction of metal ions: An alternative technology for metal regeneration

R Karmakar, K Sen - Journal of Molecular Liquids, 2019 - Elsevier
Aqueous biphasic systems (ABSs) had been perceived way back in 1896 when its mere
possibility was observed by Beijerinck. However, the immense capability of these systems …

In Aqua Veritas: The Indispensable yet Mostly Ignored Role of Water in Phase Separation and Membrane-less Organelles

BY Zaslavsky, VN Uversky - Biochemistry, 2018 - ACS Publications
Despite the common practice of presenting structures of biological molecules on an empty
background and the assumption that interactions between biological macromolecules take …

The solvent side of proteinaceous membrane-less organelles in light of aqueous two-phase systems

BY Zaslavsky, LA Ferreira, AL Darling… - International journal of …, 2018 - Elsevier
Water represents a common denominator for liquid-liquid phase transitions leading to the
formation of the polymer-based aqueous two-phase systems (ATPSs) and a set of the …

Isolation of PCR DNA fragments using aqueous two-phase systems

T Matos, HO Johansson, JA Queiroz, L Bulow - Separation and Purification …, 2014 - Elsevier
Aqueous two-phase systems have frequently been utilized as an efficient bioseparation tool
in the downstream processing of different biomolecules. The poly (ethyleneglycol)/poly …

Partitioning behavior of short DNA fragments in polymer/salt aqueous two‐phase systems

R Meutelet, LJ Bisch, BC Buerfent, M Müller… - Biotechnology …, 2024 - Wiley Online Library
The development of liquid biopsy as a minimally invasive technique for tumor profiling has
created a need for efficient biomarker extraction systems from body fluids. The analysis of …

Plasmid DNA purification using a multimodal chromatography resin

T Matos, JA Queiroz, L Bülow - Journal of Molecular …, 2014 - Wiley Online Library
Multimodal chromatography is widely used for isolation of proteins because it often results in
improved selectivity compared to conventional separation resins. The binding potential and …

Plasmid DNA affinity partitioning using polyethylene glycol–sodium sulfate aqueous two-phase systems

B Nazer, MR Dehghani, B Goliaei - Journal of Chromatography B, 2017 - Elsevier
Extraction of plasmid DNA (pDNA) from an alkaline bacterial cell lysate has been
investigated using partitioning and affinity partitioning in aqueous two-phase systems …