Technology development in biological research often aims to either increase the number of
cellular features that can be surveyed simultaneously or enhance the resolution at which …

Recent advances in flow cytometry allow scientists to measure an increasing number of
parameters per cell, generating huge and high-dimensional datasets. To analyse, visualize …

The simultaneous measurement of multiple modalities represents an exciting frontier for
single-cell genomics and necessitates computational methods that can define cellular states …

These guidelines are a consensus work of a considerable number of members of the
immunology and flow cytometry community. They provide the theory and key practical …

From a technical point of view a flow cytometer is a light detection device capable of
detecting photons of different wavelengths over a high dynamic range. In order to achieve a …

Natural killer (NK) cells are an emerging cellular immunotherapy for patients with acute
myeloid leukemia (AML); however, the best approach to maximize NK cell antileukemia …

Patients who receive chimeric antigen receptor (CAR)–T cells that are enriched in memory T
cells exhibit better disease control as a result of increased expansion and persistence of the …

Natural killer (NK) cells are innate lymphoid cells that eliminate cancer cells, produce
cytokines, and are being investigated as a nascent cellular immunotherapy. Impaired NK …

Recent technological developments in high‐dimensional flow cytometry and mass cytometry
(CyTOF) have made it possible to detect expression levels of dozens of protein markers in …

The third edition of Flow Cytometry Guidelines provides the key aspects to consider when
performing flow cytometry experiments and includes comprehensive sections describing …