Mycobacterium bovis: From Genotyping to Genome Sequencing

AMS Guimaraes, CK Zimpel - Microorganisms, 2020 - mdpi.com
Mycobacterium bovis is the main pathogen of bovine, zoonotic, and wildlife tuberculosis.
Despite the existence of programs for bovine tuberculosis (bTB) control in many regions, the …

SpoTyping: fast and accurate in silico Mycobacterium spoligotyping from sequence reads

E Xia, YY Teo, RTH Ong - Genome medicine, 2016 - Springer
SpoTyping is a fast and accurate program for in silico spoligotyping of Mycobacterium
tuberculosis isolates from next-generation sequencing reads. This novel method achieves …

Epidemiology of transmissible diseases: array hybridization and next generation sequencing as universal nucleic acid-mediated typing tools

WM Dunne Jr, H Pouseele, S Monecke… - Infection, Genetics and …, 2018 - Elsevier
The magnitude of interest in the epidemiology of transmissible human diseases is reflected
in the vast number of tools and methods developed recently with the expressed purpose to …

Development of multiplex RT-PCR with immobilized primers for identification of infectious human pneumonia pathogens

SA Lapa, RA Miftakhov, ES Klochikhina, YI Ammur… - Molecular Biology, 2021 - Springer
A prototype of a system for the detection of infectious human pneumonia pathogens based
on multiplex solid-phase reverse transcription PCR (RT-PCR) was developed. Primers were …

Emerging peak on the phylogeographic landscape of Mycobacterium tuberculosis in West Asia: definitely smoke, likely fire

I Mokrousov, E Shitikov, Y Skiba, S Kolchenko… - Molecular phylogenetics …, 2017 - Elsevier
To date, a major attention was justly given to the global lineages of Mycobacterium
tuberculosis. Here, we demonstrated an importance of the minor ones, on an example of …

Simultaneous drug resistance detection and genotyping of Mycobacterium tuberculosis using a low-density hydrogel microarray

DV Zimenkov, EV Kulagina… - Journal of …, 2016 - academic.oup.com
Background Nucleic acid amplification tests are widely used in TB diagnostics. Priority tasks
in their development consist of increasing the specificity and sensitivity of the detection of …

Comparative study of novel fluorescent cyanine nucleotides: Hybridization analysis of labeled PCR products using a biochip

VE Shershov, SA Lapa, VE Kuznetsova… - Journal of …, 2017 - Springer
This study investigated the synthesis and substrate properties of Cy5-labeled dUTP
derivatives with different substituents, linkers between the dye unit and pyrimidine …

Proteome analysis of the Mycobacterium tuberculosis Beijing B0/W148 cluster

J Bespyatykh, E Shitikov, I Butenko, I Altukhov… - Scientific reports, 2016 - nature.com
Abstract Beijing B0/W148, a “successful” clone of Mycobacterium tuberculosis, is
widespread in the Russian Federation and some countries of the former Soviet Union. Here …

Evaluation of a low-density hydrogel microarray technique for mycobacterial species identification

DV Zimenkov, EV Kulagina, OV Antonova… - Journal of clinical …, 2015 - Am Soc Microbiol
In addition to the obligatory pathogenic species of the Mycobacterium tuberculosis complex
and Mycobacterium leprae, the genus Mycobacterium also includes conditionally …

The EIMB hydrogel microarray technology: thirty years later

DA Gryadunov, BL Shaskolskiy… - Acta Naturae …, 2018 - cyberleninka.ru
Biological microarrays (biochips) are analytical tools that can be used to implement complex
integrative genomic and proteomic approaches to the solution of problems of personalized …