Environment-dependent single-chain mechanics of synthetic polymers and biomacromolecules by atomic force microscopy-based single-molecule force spectroscopy …
“The Tao begets the One. One begets all things of the world.” This quote from Tao Te Ching
is still inspiring for scientists in chemistry and materials science: The “One” can refer to a …
is still inspiring for scientists in chemistry and materials science: The “One” can refer to a …
Next generation methods for single-molecule force spectroscopy on polyproteins and receptor-ligand complexes
Single-molecule force spectroscopy with the atomic force microscope provides molecular
level insights into protein function, allowing researchers to reconstruct energy landscapes …
level insights into protein function, allowing researchers to reconstruct energy landscapes …
Combination of click chemistry and enzymatic ligation for stable and efficient protein immobilization for single-molecule force spectroscopy
S Shi, Z Wang, Y Deng, F Tian, Q Wu… - CCS Chemistry, 2022 - chinesechemsoc.org
Protein immobilization is an essential method for both basic and applied research for
protein, and covalent, site-specific attachment is the most desirable strategy. Classic …
protein, and covalent, site-specific attachment is the most desirable strategy. Classic …
Enzymatic biosynthesis and immobilization of polyprotein verified at the single-molecule level
The recent development of chemical and bio-conjugation techniques allows for the
engineering of various protein polymers. However, most of the polymerization process is …
engineering of various protein polymers. However, most of the polymerization process is …
Histidine-specific bioconjugation for single-molecule force spectroscopy
Atomic force microscopy (AFM) based single-molecule force spectroscopy (SMFS) is a
powerful tool to study the mechanical properties of proteins. In these experiments, site …
powerful tool to study the mechanical properties of proteins. In these experiments, site …
Mechanisms of nanonewton mechanostability in a protein complex revealed by molecular dynamics simulations and single-molecule force spectroscopy
Can molecular dynamics simulations predict the mechanical behavior of protein complexes?
Can simulations decipher the role of protein domains of unknown function in large …
Can simulations decipher the role of protein domains of unknown function in large …
Broadening the scope of sortagging
X Dai, A Böker, U Glebe - RSC advances, 2019 - pubs.rsc.org
Sortases are enzymes occurring in the cell wall of Gram-positive bacteria. Sortase A (SrtA),
the best studied sortase class, plays a key role in anchoring surface proteins with the …
the best studied sortase class, plays a key role in anchoring surface proteins with the …
Direct Comparison of Lysine versus Site‐Specific Protein Surface Immobilization in Single‐Molecule Mechanical Assays
Single‐molecule force spectroscopy (SMFS) is powerful for studying folding states and
mechanical properties of proteins, however, it requires protein immobilization onto force …
mechanical properties of proteins, however, it requires protein immobilization onto force …
Removal of a conserved disulfide bond does not compromise mechanical stability of a VHH antibody complex
Single-domain VHH antibodies are promising reagents for medical therapy. A conserved
disulfide bond within the VHH framework region is known to be critical for thermal stability …
disulfide bond within the VHH framework region is known to be critical for thermal stability …
Extreme mechanical stability in protein complexes
Highlights•Non-covalent protein–protein interactions with rupture forces larger than 2000
pN.•Mechanical stability of protein complexes is critically dependent on force application …
pN.•Mechanical stability of protein complexes is critically dependent on force application …