Principles of genetic circuit design
JAN Brophy, CA Voigt - Nature methods, 2014 - nature.com
Cells navigate environments, communicate and build complex patterns by initiating gene
expression in response to specific signals. Engineers seek to harness this capability to …
expression in response to specific signals. Engineers seek to harness this capability to …
Genome-editing technologies: principles and applications
Targeted nucleases have provided researchers with the ability to manipulate virtually any
genomic sequence, enabling the facile creation of isogenic cell lines and animal models for …
genomic sequence, enabling the facile creation of isogenic cell lines and animal models for …
Editing the epigenome: technologies for programmable transcription and epigenetic modulation
Gene regulation is a complex and tightly controlled process that defines cell identity, health
and disease, and response to pharmacologic and environmental signals. Recently …
and disease, and response to pharmacologic and environmental signals. Recently …
Multiplex CRISPR/Cas9-based genome engineering from a single lentiviral vector
AM Kabadi, DG Ousterout, IB Hilton… - Nucleic acids …, 2014 - academic.oup.com
Engineered DNA-binding proteins that manipulate the human genome and transcriptome
have enabled rapid advances in biomedical research. In particular, the RNA-guided …
have enabled rapid advances in biomedical research. In particular, the RNA-guided …
Techniques for investigating lncRNA transcript functions in neurodevelopment
T Srinivas, E Siqueira, S Guil - Molecular Psychiatry, 2024 - nature.com
Long noncoding RNAs (lncRNAs) are sequences of 200 nucleotides or more that are
transcribed from a large portion of the mammalian genome. While hypothesized to have a …
transcribed from a large portion of the mammalian genome. While hypothesized to have a …
Genome-wide specificity of DNA binding, gene regulation, and chromatin remodeling by TALE-and CRISPR/Cas9-based transcriptional activators
LR Polstein, P Perez-Pinera, DD Kocak… - Genome …, 2015 - genome.cshlp.org
Genome engineering technologies based on the CRISPR/Cas9 and TALE systems are
enabling new approaches in science and biotechnology. However, the specificity of these …
enabling new approaches in science and biotechnology. However, the specificity of these …
Engineering synthetic gene circuits in living cells with CRISPR technology
One of the goals of synthetic biology is to build regulatory circuits that control cell behavior,
for both basic research purposes and biomedical applications. The ability to build …
for both basic research purposes and biomedical applications. The ability to build …
Synthetic zinc finger proteins: the advent of targeted gene regulation and genome modification technologies
Conspectus The understanding of gene regulation and the structure and function of the
human genome increased dramatically at the end of the 20th century. Yet the technologies …
human genome increased dramatically at the end of the 20th century. Yet the technologies …
Enabling tools for high-throughput detection of metabolites: metabolic engineering and directed evolution applications
Abstract Within the Design-Build-Test Cycle for strain engineering, rapid product detection
and selection strategies remain challenging and limit overall throughput. Here we …
and selection strategies remain challenging and limit overall throughput. Here we …
The multi-faceted potential of plant-derived metabolites as antimicrobial agents against multidrug-resistant pathogens
J Shin, VS Prabhakaran, K Kim - Microbial pathogenesis, 2018 - Elsevier
Multidrug-resistant (MDR) pathogens are currently causing serious problems globally in the
medical setting. Improper and extensive usage of antibiotics results in a selective pressure …
medical setting. Improper and extensive usage of antibiotics results in a selective pressure …