Viral Vectors for the in Vivo Delivery of CRISPR Components: Advances and Challenges

M Asmamaw Mengstie - Frontiers in Bioengineering and …, 2022 - frontiersin.org
The Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR) and its
accompanying protein (Cas9) are now the most effective, efficient, and precise genome …

[HTML][HTML] The present and potential future methods for delivering CRISPR/Cas9 components in plants

D Sandhya, P Jogam, VR Allini, S Abbagani… - Journal of Genetic …, 2020 - Elsevier
Background CRISPR/Cas9 genome editing technology is a DNA manipulation tool for trait
improvement. This technology has been demonstrated and successfully applied to edit the …

Viral vectors and extracellular vesicles: innate delivery systems utilized in CRISPR/Cas-mediated cancer therapy

SE Ahmadi, M Soleymani, F Shahriyary… - Cancer Gene …, 2023 - nature.com
Gene editing-based therapeutic strategies grant the power to override cell machinery and
alter faulty genes contributing to disease development like cancer. Nowadays, the principal …

Expanding the plant genome editing toolbox with recently developed CRISPR–Cas systems

N Wada, K Osakabe, Y Osakabe - Plant Physiology, 2022 - academic.oup.com
Since its first appearance, CRISPR–Cas9 has been developed extensively as a
programmable genome-editing tool, opening a new era in plant genome engineering …

CRISPR-Cas: A robust technology for enhancing consumer-preferred commercial traits in crops

V Verma, A Kumar, M Partap, M Thakur… - Frontiers in Plant …, 2023 - frontiersin.org
The acceptance of new crop varieties by consumers is contingent on the presence of
consumer-preferred traits, which include sensory attributes, nutritional value, industrial …

Systematic optimization of Cas12a base editors in wheat and maize using the ITER platform

C Gaillochet, A Peña Fernández, V Goossens… - Genome biology, 2023 - Springer
Background Testing an ever-increasing number of CRISPR components is challenging
when developing new genome engineering tools. Plant biotechnology has few high …

Guide RNAs containing universal bases enable Cas9/Cas12a recognition of polymorphic sequences

AR Krysler, CR Cromwell, T Tu, J Jovel… - Nature …, 2022 - nature.com
CRISPR/Cas complexes enable precise gene editing in a wide variety of organisms. While
the rigid identification of DNA sequences by these systems minimizes the potential for off …

Genetic and covalent protein modification strategies to facilitate intracellular delivery

JM Horn, AC Obermeyer - Biomacromolecules, 2021 - ACS Publications
Protein-based therapeutics represent a rapidly growing segment of approved disease
treatments. Successful intracellular delivery of proteins is an important precondition for …

Accelerating wood domestication in forest trees through genome editing: Advances and prospects

C Anders, L Hoengenaert, W Boerjan - Current opinion in plant biology, 2023 - Elsevier
The high economic value of wood requires intensive breeding towards multipurpose
biomass. However, long breeding cycles hamper the fast development of novel tree varieties …

Expert opinions on the regulation of plant genome editing

R Lassoued, PWB Phillips, DM Macall… - Plant Biotechnology …, 2021 - Wiley Online Library
Global food security is largely affected by factors such as environmental (eg drought,
flooding), social (eg gender inequality), socio‐economic (eg overpopulation, poverty) and …