Cryogenic electron microscopy and data processing enable the determination of structures
of isolated macromolecules to near-atomic resolution. However, these data do not provide …

Structural biologists have traditionally approached cellular complexity in a reductionist
manner in which the cellular molecular components are fractionated and purified before …

Actin dynamics in cell motility, division, and phagocytosis is regulated by complex factors
with multiple feedback loops, often leading to emergent dynamic patterns in the form of …

The actin-related protein (Arp) 2/3 complex nucleates branched actin filament networks
pivotal for cell migration, endocytosis and pathogen infection. Its activation is tightly …

Macropinocytosis is a conserved endocytic process by which cells engulf droplets of
medium into micron-sized vesicles. We use light-sheet microscopy to define an underlying …

Actin assembly provides force for a multitude of cellular processes. Compared to actin-
assembly-based force production during cell migration, relatively little is understood about …

Actin filaments (F-actin) are a key component of eukaryotic cells. Whether serving as a
scaffold for myosin or using their polymerization to push onto cellular components, their …

Cryo-electron tomography has stepped fully into the spotlight. Enthusiasm is high.
Fortunately for us, this is an exciting time to be a cryotomographer, but there is still a way to …

During activation the platelet cytoskeleton is reorganized, inducing adhesion to the
extracellular matrix and cell spreading. These processes are critical for wound healing and …

A precise quantitative description of the ultrastructural characteristics underlying biological
mechanisms is often key to their understanding. This is particularly true for dynamic extra …