Label-Free Techniques for Probing Biomolecular Condensates

KA Ibrahim, AS Naidu, H Miljkovic, A Radenovic… - ACS …, 2024 - ACS Publications
Biomolecular condensates play important roles in a wide array of fundamental biological
processes, such as cellular compartmentalization, cellular regulation, and other biochemical …

Liquid-liquid phase separation in human diseases: Functions, mechanisms and treatments

T Yue, F Zhang, Y Wei, Z Wang - Nano Today, 2024 - Elsevier
Cells are separated into functional regions by non-membrane organelles and membrane-
enclosed organelles, ensuring that various cellular activities occur in a controlled manner in …

Emulsion imaging of a DNA nanostar condensate phase diagram reveals valence and electrostatic effects

N Conrad, G Chang, DK Fygenson… - The Journal of Chemical …, 2022 - pubs.aip.org
Liquid–liquid phase separation (LLPS) in macromolecular solutions (eg, coacervation) is
relevant both to technology and to the process of mesoscale structure formation in cells. The …

Nucleation of biomolecular condensates from finite-sized simulations

L Li, M Paloni, AR Finney, A Barducci… - The Journal of …, 2023 - ACS Publications
The nucleation of protein condensates is a concentration-driven process of assembly. When
modeled in the canonical ensemble, condensation is affected by finite-size effects. Here, we …

Aqueous two-phase systems: Methods of binodal curve generation and applications

NN Areekal, JAR Boravelli, K Raghavarao… - Advances in Colloid and …, 2025 - Elsevier
Aqueous two-phase system (ATPS) has been of interest to both industry and academia for
the extraction and purification of biomolecules/bioactives. ATPSs are formed by two-phase …

Oxygen-co-Thermal Driver for Constructing Integrated Anisotropic Hydrogels with Programmable Shape Deformation, Superior Self-Healing, and Mechanical …

S Ye, W Yong, J Xu, G Fu - Chemistry of Materials, 2023 - ACS Publications
Gradient hydrogels hold great potential for applications in actuators due to their unique
stimulus response performance and simple integrated structure. However, most of the …

Quantification of the concentration in a droplet formed by liquid–liquid phase separation of G-quadruplex-forming RNA

K Yokosawa, M Tsuruta, S Kajimoto, N Sugimoto… - Chemical Physics …, 2023 - Elsevier
Liquid–liquid phase separation (LLPS), which results in the formation of highly concentrated
droplets of biomolecules, is involved in various physiological phenomena. We have …

Emerging experimental methods to study the thermodynamics of biomolecular condensate formation

S Ray, AK Buell - The Journal of Chemical Physics, 2024 - pubs.aip.org
The formation of biomolecular condensates in vivo is increasingly recognized to underlie a
multitude of crucial cellular functions. Furthermore, the evolution of highly dynamic protein …

Continuity of Short-Time Dynamics Crossing the Liquid–Liquid Phase Separation in Charge-Tuned Protein Solutions

I Mosca, C Beck, NH Jalarvo… - The Journal of …, 2024 - ACS Publications
Liquid–liquid phase separation (LLPS) constitutes a crucial phenomenon in biological self-
organization, not only intervening in the formation of membraneless organelles but also …

Taylor Dispersion‐Induced Phase Separation for the Efficient Characterisation of Protein Condensate Formation

RK Norrild, TO Mason, L Boyens‐Thiele… - Angewandte …, 2024 - Wiley Online Library
Biomolecular condensates have emerged as important structures in cellular function and
disease, and are thought to form through liquid‐liquid phase separation (LLPS). Thorough …