Over the last few decades, mass spectrometry‐based proteomics has become an
increasingly powerful tool that is now able to routinely detect and quantify thousands of …

We discuss the evolution of OrbitrapTM mass spectrometry (MS) from its birth in the late
1990s to its current role as one of the most prominent techniques for MS. The Orbitrap mass …

Isobaric labeling empowers proteome-wide expression measurements simultaneously
across multiple samples. Here an expanded set of 16 isobaric reagents based on an …

Single-cell proteomics by mass spectrometry (SCoPE-MS) is a recently introduced method
to quantify multiplexed single-cell proteomes. While this technique has generated great …

The proteome is a dynamic system in which each protein has interconnected properties—
dimensions—that together contribute to the phenotype of a cell. Measuring these properties …

We describe the comprehensive analysis of the yeast proteome in just over one hour of
optimized analysis. We achieve this expedited proteome characterization with improved …

Several mechanisms have been proposed for histone marks to exert their functions,
including altering the physical properties of nucleosomes by neutralization of charge via …

Protein phosphorylation is a post-translational modification (PTM) that orchestrates a diverse
array of cellular processes. Because this modification serves as a rapid and reversible …

SILAC is based on direct addition of selected stable isotope amino acids into the cell culture
medium, allowing superior quantitative analysis of the cellular proteome compared to other …

Mass spectrometry (MS)–based high-throughput proteomics is the core technique for large-
scale protein characterization. Due to the extreme complexity of proteomes, sophisticated …