Purification of cross-linked RNA-protein complexes by phenol-toluol extraction

EC Urdaneta, CH Vieira-Vieira, T Hick… - Nature …, 2019 - nature.com
Recent methodological advances allowed the identification of an increasing number of RNA-
binding proteins (RBPs) and their RNA-binding sites. Most of those methods rely, however …

Regulation of 6S RNA by pRNA synthesis is required for efficient recovery from stationary phase in E. coli and B. subtilis

AT Cavanagh, JM Sperger… - Nucleic acids …, 2012 - academic.oup.com
Abstract 6S RNAs function through interaction with housekeeping forms of RNA polymerase
holoenzyme (Eσ70 in Escherichia coli, EσA in Bacillus subtilis). Escherichia coli 6S RNA …

Determination of sRNA Expressions by RNA-seq in Yersinia pestis Grown In Vitro and during Infection

Y Yan, S Su, X Meng, X Ji, Y Qu, Z Liu, X Wang, Y Cui… - PloS one, 2013 - journals.plos.org
Background Small non-coding RNAs (sRNAs) facilitate host-microbe interactions. They have
a central function in the post-transcriptional regulation during pathogenic lifestyles. Hfq, an …

A pRNA‐induced structural rearrangement triggers 6S‐1 RNA release from RNA polymerase in Bacillus subtilis

BM Beckmann, PG Hoch, M Marz, DK Willkomm… - The EMBO …, 2012 - embopress.org
Bacillus subtilis 6S‐1 RNA binds to the housekeeping RNA polymerase (σA‐RNAP) and
directs transcription of short 'product'RNAs (pRNAs). Here, we demonstrate that once newly …

[HTML][HTML] Two sRNA RyhB homologs from Yersinia pestis biovar microtus expressed in vivo have differential Hfq-dependent stability

Z Deng, X Meng, S Su, Z Liu, X Ji, Y Zhang… - Research in …, 2012 - Elsevier
Small non-coding RNAs (sRNAs) have been shown to modulate gene expression at the post-
transcriptional level. RyhB, an iron-responsive sRNA, is conserved in Escherichia coli and …

In vivo and in vitro analysis of 6S RNA-templated short transcripts in Bacillus subtilis

BM Beckmann, OY Burenina, PG Hoch… - RNA biology, 2011 - Taylor & Francis
By differential high-throughput RNA sequencing (dRNA-seq) we have identified “product
RNAs”(pRNAs) as short as 8-12 nucleotides that are synthesized by Bacillus subtilis RNA …

A simple and efficient method to search for selected primary transcripts: non-coding and antisense RNAs in the human pathogen Enterococcus faecalis

A Fouquier d'Hérouel, F Wessner… - Nucleic acids …, 2011 - academic.oup.com
Enterococcus faecalis is a commensal bacterium and a major opportunistic human
pathogen. In this study, we combined in silico predictions with a novel 5′ RACE-derivative …

Involvement of E. coli 6S RNA in Oxidative Stress Response

OY Burenina, DA Elkina, A Ovcharenko… - International Journal of …, 2022 - mdpi.com
6S RNA, a small non-coding RNA present in almost all bacteria, inhibits transcription via
direct binding to RNA polymerase holoenzymes. The mechanism of 6S RNA action was …

6S-1 RNA function leads to a delay in sporulation in Bacillus subtilis

AT Cavanagh, KM Wassarman - Journal of bacteriology, 2013 - Am Soc Microbiol
We have discovered that 6S-1 RNA (encoded by bsrA) is important for appropriate timing of
sporulation in Bacillus subtilis in that cells lacking 6S-1 RNA sporulate earlier than wild-type …

Impact of RNA isolation protocols on RNA detection by Northern blotting

K Damm, S Bach, KMH Müller, G Klug… - Small Non-Coding …, 2015 - Springer
We prepared total RNA from the Gram-positive soil bacterium Bacillus subtilis by different
RNA extraction procedures to compare their suitability for Northern blot detection of tiny …