Phase-separating RNA-binding proteins form heterogeneous distributions of clusters in subsaturated solutions
Macromolecular phase separation is thought to be one of the processes that drives the
formation of membraneless biomolecular condensates in cells. The dynamics of phase …
formation of membraneless biomolecular condensates in cells. The dynamics of phase …
Ligand effects on phase separation of multivalent macromolecules
Biomolecular condensates enable spatial and temporal control over cellular processes by
concentrating biomolecules into nonstoichiometric assemblies. Many condensates form via …
concentrating biomolecules into nonstoichiometric assemblies. Many condensates form via …
Chromatin accessibility in the Drosophila embryo is determined by transcription factor pioneering and enhancer activation
KJ Brennan, M Weilert, S Krueger, A Pampari, H Liu… - Developmental cell, 2023 - cell.com
Chromatin accessibility is integral to the process by which transcription factors (TFs) read out
cis-regulatory DNA sequences, but it is difficult to differentiate between TFs that drive …
cis-regulatory DNA sequences, but it is difficult to differentiate between TFs that drive …
RNA polymerase II clusters form in line with surface condensation on regulatory chromatin
It is essential for cells to control which genes are transcribed into RNA. In eukaryotes, two
major control points are recruitment of RNA polymerase II (Pol II) into a paused state, and …
major control points are recruitment of RNA polymerase II (Pol II) into a paused state, and …
Co-condensation of proteins with single-and double-stranded DNA
R Renger, JA Morin, R Lemaitre… - Proceedings of the …, 2022 - National Acad Sciences
Biomolecular condensates provide distinct compartments that can localize and organize
biochemistry inside cells. Recent evidence suggests that condensate formation is prevalent …
biochemistry inside cells. Recent evidence suggests that condensate formation is prevalent …
FUS oncofusion protein condensates recruit mSWI/SNF chromatin remodeler via heterotypic interactions between prion‐like domains
Fusion transcription factors generated by genomic translocations are common drivers of
several types of cancers including sarcomas and leukemias. Oncofusions of the FET (FUS …
several types of cancers including sarcomas and leukemias. Oncofusions of the FET (FUS …
HP1-driven phase separation recapitulates the thermodynamics and kinetics of heterochromatin condensate formation
MMC Tortora, LD Brennan… - Proceedings of the …, 2023 - National Acad Sciences
The spatial segregation of pericentromeric heterochromatin (PCH) into distinct, membrane-
less nuclear compartments involves the binding of Heterochromatin Protein 1 (HP1) to …
less nuclear compartments involves the binding of Heterochromatin Protein 1 (HP1) to …
Chromatin sequesters pioneer transcription factor Sox2 from exerting force on DNA
Biomolecular condensation constitutes an emerging mechanism for transcriptional
regulation. Recent studies suggest that the co-condensation between transcription factors …
regulation. Recent studies suggest that the co-condensation between transcription factors …
Thermodynamics of wetting, prewetting and surface phase transitions with surface binding
In living cells, protein-rich condensates can wet the cell membrane and surfaces of
membrane-bound organelles. Interestingly, many phase-separating proteins also bind to …
membrane-bound organelles. Interestingly, many phase-separating proteins also bind to …
Surface densities prewet a near critical membrane
Recent work has highlighted roles for thermodynamic phases in cellular processes. Proteins
and nucleic acids can phase separate into three-dimensional liquid droplets in the …
and nucleic acids can phase separate into three-dimensional liquid droplets in the …