Handmade cloning (HMC) is the most awaited, simple and micromanipulator-free version of
somatic cell nuclear transfer (SCNT). The requirement of expensive micromanipulators and …

The Bonin flying fox (Pteropus pselaphon) is one of the most critically endangered species
of animals. The number of this species is estimated to be around 150; being classified at the …

The objective of this study was to examine the effect of cytochalasin B (CB) and/or
demecolcine (Dc) on the remodeling of donor nuclei, nuclear ploidy, and development of …

Despite its success in almost all farm and laboratory animals, somatic cell nuclear transfer
(SCNT) is still a low-efficiency technique. In this investigation, we determined the impact of …

We examined the effects of the source of recipient oocytes and timing of fusion and
activation on the development competence of bovine nuclear transferred (NT) embryos …

Demecolcine (DEM) treatment of oocytes induces formation of a membrane protrusion
containing a mass of condensed maternal chromosomes, which can be removed with …

Although demecolcine-assisted enucleation has been performed successfully in porcine
and cattle, the mechanism and protocol optimization of chemically assisted enucleation …

This study aimed to evaluate the effect of demecolcine, a microtubule-depolymerizing agent,
on microtubule kinetics; to determine the best concentration of demecolcine as a chemically …

The removal of chromosomes from recipient oocytes is one of the key steps in nuclear
transfer cloning. Although microtubule interrupters have been successfully used for oocyte …

Mouse recipient cytoplasts for somatic cell nuclear transfer (SCNT) are routinely prepared by
mechanical enucleation (ME), an invasive procedure that requires expensive equipment …