Fluorescence lifetime imaging (FLIM): Basic concepts and some recent developments

K Suhling, LM Hirvonen, JA Levitt, PH Chung… - Medical photonics, 2015 - Elsevier
Fluorescence lifetime imaging (FLIM) is a key fluorescence microscopy technique to map the
environment and interaction of fluorescent probes. It can report on photophysical events that …

Fluorescence anisotropy (polarization): from drug screening to precision medicine

H Zhang, Q Wu, MY Berezin - Expert opinion on drug discovery, 2015 - Taylor & Francis
Introduction: Fluorescence anisotropy (FA) is one of the major established methods
accepted by industry and regulatory agencies for understanding the mechanisms of drug …

A self-calibrating bipartite viscosity sensor for mitochondria

Z Yang, Y He, JH Lee, N Park, M Suh… - Journal of the …, 2013 - ACS Publications
A self-calibrating bipartite viscosity sensor 1 for cellular mitochondria, composed of
coumarin and boron-dipyrromethene (BODIPY) with a rigid phenyl spacer and a …

Mapping viscosity in cells using molecular rotors

MK Kuimova - Physical Chemistry Chemical Physics, 2012 - pubs.rsc.org
This article describes an emerging method for quantitative measurement and spatial
imaging of microviscosity within individual domains of live cells. The method is based on …

Highly Twisted N,N-Dialkylamines as a Design Strategy to Tune Simple Aromatic Hydrocarbons as Steric Environment-Sensitive Fluorophores

S Sasaki, S Suzuki, WMC Sameera… - Journal of the …, 2016 - ACS Publications
The steric-environment sensitivity of fluorescence of 9, 10-bis (N, N-dialkylamino)
anthracenes (BDAA s) was studied experimentally and theoretically. A new design strategy …

Compositional adaptability in NPM1-SURF6 scaffolding networks enabled by dynamic switching of phase separation mechanisms

MC Ferrolino, DM Mitrea, JR Michael… - Nature …, 2018 - nature.com
The nucleolus, the site for ribosome biogenesis contains hundreds of proteins and several
types of RNA. The functions of many non-ribosomal nucleolar proteins are poorly …

Exploring viscosity, polarity and temperature sensitivity of BODIPY-based molecular rotors

A Vyšniauskas, I López-Duarte, N Duchemin… - Physical Chemistry …, 2017 - pubs.rsc.org
Microviscosity is a key parameter controlling the rate of diffusion and reactions on the
microscale. One of the most convenient tools for measuring microviscosity is by fluorescent …

Insight on the Intracellular Supramolecular Assembly of DTTO: A Peculiar Example of Cell‐Driven Polymorphism

L Aloisio, M Moschetta, A Boschi, AG Fleitas… - Advanced …, 2023 - Wiley Online Library
The assembly of supramolecular structures within living systems is an innovative approach
for introducing artificial constructs and developing biomaterials capable of influencing and/or …

Electrochemical switching of a fluorescent molecular rotor embedded within a bistable rotaxane

Y Wu, M Frasconi, WG Liu, RM Young… - Journal of the …, 2020 - ACS Publications
We report how the nanoconfined environment, introduced by the mechanical bonds within
an electrochemically switchable bistable [2] rotaxane, controls the rotation of a fluorescent …

Targeted fluorescence lifetime probes reveal responsive organelle viscosity and membrane fluidity

IE Steinmark, AL James, PH Chung, PE Morton… - PLoS …, 2019 - journals.plos.org
The only way to visually observe cellular viscosity, which can greatly influence biological
reactions and has been linked to several human diseases, is through viscosity imaging …