We have elaborated a method for the isolation of ribosomal subunits from fresh unfrozen
human placenta containing intact rRNA and a complete set of ribosomal proteins. Activity of …

Publisher Summary This chapter describes a highly reproducible method for preparing
ribosomes and ribosomal subunits from a variety of eukaryotic cells. The number of sources …

The chemical analysis and CsCl buoyant density gradient centrifugation showed that the
protein content of the 32-S ribosomal subunit, prepared from rat liver ribosomes by EDTA …

Human placenta and Escherichia coli Phe-tRNA Phe and N-AcPhe-tRNA Phe binding to
human placenta 80S ribosomes was studied at 13 mM Mg 2+ and 20° C in the presence of …

Ribosomal subunits from rat liver were prepared with a mild method which consisted of
centrifuging ribosomes through a sucrose layer containing 0.3 M KCl. Isolated 40‐S subunits …

We have developed a method for preparation and purification of active ribosomal subunits
from rat and mouse liver. Our technique involves incubating purified polysomes with all …

Rat liver ribosomes were dissociated to 60S and 40S fractions by incubation with 1 M KCl at
37° for 2 h, and separated by subsequent density-gradient centrifugation. While the 40S …

HE importance of ribosomes to protein biosyn-thesis in both bacterial and animal cells is
now well established". In cell-free systems, consisting essentially of ribosomes, amino-acid …

Highly active salt-washed ribosomes from Escherichia coli are isolated by gel filtration on
Sephacryl S-200 in a buffer containing 1 m ammonium chloride and putrescine, spermidine …

Proteins were isolated from 80-S preribosomal particles and ribosomal subunits of murine
L5178Y cells after short and longer periods of incubation with tritiated amino acids. The …