Labeled microRNA pull-down assay system: an experimental approach for high-throughput identification of microRNA-target mRNAs
RJ Hsu, HJ Yang, HJ Tsai - Nucleic acids research, 2009 - academic.oup.com
We developed a simple, direct and cost-effective approach to search for the most likely
target genes of a known microRNA (miRNA) in vitro. We term this method 'labeled miRNA …
target genes of a known microRNA (miRNA) in vitro. We term this method 'labeled miRNA …
Performing the Labeled microRNA pull-down (LAMP) assay system: an experimental approach for high-throughput identification of microRNA-target mRNAs
RJ Hsu, HJ Tsai - Therapeutic Oligonucleotides: Methods and Protocols, 2011 - Springer
We developed a simple, direct, and cost-effective approach to search for the most likely
target genes of a known miRNA in vitro. We term this method “Labeled microRNA (miRNA) …
target genes of a known miRNA in vitro. We term this method “Labeled microRNA (miRNA) …
A novel biochemical method to identify target genes of individual microRNAs: identification of a new Caenorhabditis elegans let-7 target
Y Andachi - RNA, 2008 - rnajournal.cshlp.org
MicroRNAs (miRNAs) are roughly 22-nucleotide regulatory RNAs that play important roles in
many developmental and physiological processes. Animal miRNAs down-regulate target …
many developmental and physiological processes. Animal miRNAs down-regulate target …
A quantitative method to identify microRNAs targeting a messenger RNA using a 3′ UTR RNA affinity technique
M Shi, W Han, SD Spivack - Analytical biochemistry, 2013 - Elsevier
The identification of specific microRNAs (miRNAs) that target a given messenger RNA
(mRNA) is essential for studies in gene regulation, but the available bioinformatic software …
(mRNA) is essential for studies in gene regulation, but the available bioinformatic software …
A functional assay for microRNA target identification and validation
J Gäken, AM Mohamedali, J Jiang, F Malik… - Nucleic acids …, 2012 - academic.oup.com
MicroRNAs (miRNA) are a class of small RNA molecules that regulate numerous critical
cellular processes and bind to partially complementary sequences resulting in down …
cellular processes and bind to partially complementary sequences resulting in down …
Detecting microRNA targets by linking sequence, microRNA and gene expression data
MicroRNAs (miRNAs) have recently been discovered as an important class of non-coding
RNA genes that play a major role in regulating gene expression, providing a means to …
RNA genes that play a major role in regulating gene expression, providing a means to …
A novel method to detect functional microRNA targets
S Vatolin, K Navaratne, RJ Weil - Journal of molecular biology, 2006 - Elsevier
MicroRNA (miRNA) molecules are non-coding RNAs, 19 to 24nt in length that have been
identified recently as important regulators of gene expression. Several computational …
identified recently as important regulators of gene expression. Several computational …
Strategies to identify microRNA targets: new advances
H Jin, W Tuo, H Lian, Q Liu, XQ Zhu, H Gao - New Biotechnology, 2010 - Elsevier
MicroRNAs (miRNAs) are small regulatory RNA molecules functioning to modulate gene
expression at the post-transcriptional level, and playing an important role in many …
expression at the post-transcriptional level, and playing an important role in many …
Experimental validation of microRNA targets using a luciferase reporter system
FE Nicolas - MicroRNAs in Development: Methods and Protocols, 2011 - Springer
MicroRNAs (miRNAs) are a class of small noncoding transcripts that repress gene
expression by pairing with their target messenger RNAs (mRNAs). The human genome …
expression by pairing with their target messenger RNAs (mRNAs). The human genome …
Biotin-based pulldown assay to validate mRNA targets of cellular miRNAs
MicroRNAs (miRNAs) are a class of small noncoding RNAs that post-transcriptionally
regulate cellular gene expression. MiRNAs bind to the 3'untranslated region (UTR) of target …
regulate cellular gene expression. MiRNAs bind to the 3'untranslated region (UTR) of target …